响应面法优化白肛海地瓜内脏团制备抗氧化肽的酶解工艺  

Screening for Optimal Enzyme for Preparation of the Acaudina leucoprocta Protein Hydrolysate with Antixodiant Activities and Process Optimization by Response Surface Methodology

作　　者：袁坤山[1] 郑艺[1] 于跃芹[1] 王雷[2] 王宝杰[2] 

机构地区：[1]青岛科技大学,青岛266042 [2]中国科学院海洋研究所,青岛266071

出　　处：《食品工业》2014年第12期37-41,共5页The Food Industry

基　　金：国家自然科学基金资助项目(20876081)

摘　　要：以白肛海地瓜蛋白为原料,选用碱性蛋白酶、复合蛋白酶、风味蛋白酶、木瓜蛋白酶和中性蛋白酶分别对其进行酶解,采用DPPH法对酶解产物进行抗氧化活性分析,筛选出酶解产物具有较强抗氧化活性的木瓜蛋白酶作为最适用酶,然后通过响应面法对酶解工艺条件进行了优化。结果表明,木瓜蛋白酶酶解白肛海地瓜的最佳酶解参数为:温度为60℃,pH为6.3,干基质加酶量质量分数为2.16%,酶解时间为5 h,此时3 mg/mL酶解物的还原能力为43.099%,试验验证结果为43.538%。响应面法预测值与试验验证结果吻合性良好。此外,最佳酶解条件下所得酶解产物的氨基酸含量丰富,甘氨酸含量较多,分子量在2 000 Da以下的组分占总量的63.09%。The protein hydrolysates of Acaudina leucoprocta were prepared with alcalase, protamex, flavourzyme, neutrase and papain, respectively. Their DPPH radical scavenging activity（DRSA） was comparatively determined by the DPPH method, with the result that hydrolyzing Acaudina leucoprocta protein by Papain to a hydrolysate with better DRSA. The optimum technological parameters for preparing antioxidant peptide with papain were obtained as follows： pH 6.3 at 60 ℃, enzyme to substrate ratio 2.16% and the hydrolysis time 5 h. Under this condition, the DRSA of 3 mg/mL hydrolysates was 43.099%, and the experimental result was 43.538%. The experimental results were good agreement with the predicted value. Furthermore, glycine was the main amino acids in the enzymatic hydrolysis product, and the components molecular weight under 2 000 Da account for 63.09% of the total enzymatic hydrolysis product.

关 键 词：白肛海地瓜 抗氧化活性 响应面分析法 酶解 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]