HPLC法同时测定植物油中维生素A和不同构型维生素E含量  

Simultaneous determination of vitamin A and different configuration of vitamin E in vegetable oils by HPLC

作　　者：姜波[1] 胡文忠[1] 刘长建[1] 姜爱丽[1] 王艳颖[1] 高琳[1] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116600

出　　处：《食品工业科技》2015年第3期320-323,334,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(31172009)资助;中央高校基本科研业务费专项资金资助项目(DC110110)

摘　　要：以石油醚为溶剂，用索氏提取法提取了黑芝麻、白花生、黑花生、葵花籽、大豆、棉花籽的油脂。油样品先用正己烷溶解，然后用甲醇定容，其中正己烷含量为20％（v／v），用Hypersil ODS2 5μm色谱柱，甲醇为流动相，对所提油脂中维生素A、α-维生素E、γ-（β）维生素E、δ-维生素E舍量进行检测。实验结果表明，在204nm波长下，维生素A、α-维生素E、γ-（β）维生素E、δ-维生素E在0-200μg／mL范围内与峰面积有良好的线性关系，标准曲线相关系数。均大于0．9978。维生素A、α-维生素E、γ-（β）维生素E、δ-维生素E加标平均回收率分别为97．83％、98．58％、98．75％、99．93％。Oils were extracted from white semen sesami nigrum, white peanut, black peanut, sunflower seed, soybean and cotton seed by Soxhlet extraction with mineral ether.Oil samples were dissolved with normal hexane and being a constant volume with methyl alcohol,the content of normal hexane was 20% （v/v）in the solution.The contents of vitamin A,vitamin α-E,vitamin Y-（β）E,vitamin δ-E in the extracted oils were determined on a ODS2 column, using methyl alcohol as mobile phase. Experimental results showed that linear relations of the concentrations of vitamin A, vitamin α-E, vitamin γ-（β） E, vitamin δ-E to peak areas were obtained within 0-200μg/mL at 204nm.The correlation coefficients were more than 0.9978.The average recoveries of vitamin A, vitamin α-E,vitamin γ-（β） E,vitamin δ-E were 97.83% ,98.58% ,98.75% and 99.93% ,respectively.

关 键 词：HPLC 植物油 维生素A 不同构型维生素E 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]