复合酶制剂在玉米原料酒精发酵中的应用  

Application of Compound Enzyme in Alcohol Fermentation from Corn

作　　者：彭伟林 刘国锋 王颖[1] 郭学武[1] 陈叶福[1] 肖冬光[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]深圳市彩田学校,广东深圳518036 [3]湖南尤特尔生化有限公司,湖南岳阳414009

出　　处：《酿酒科技》2015年第2期69-72,75,共5页Liquor-Making Science & Technology

基　　金：十二五农村领域国家科技计划(同步生物加工法制备纤维素乙醇技术研究;项目号:2012AA101805)资助

摘　　要：研究了复合酶制剂在玉米原料酒精发酵中的应用,经优化后木聚糖酶、纤维素酶和酸性蛋白酶最适添加量为:木聚糖酶170 U/g原料、纤维素酶16 U/g原料、酸性蛋白酶14 U/g原料,料水比为1∶2.5,发酵培养基初始p H5.5,3种酶制剂的最优添加时间为:木聚糖酶、酸性蛋白酶糖化前添加,纤维素酶发酵前添加。此时,原料出酒率达到44.54%,发酵周期缩短12 h。The application of compound enzyme in alcohol fermentation from corn was investigated in this paper. The results showed that, the optimum adding levels of xylanase, cellulase and acid protease were 170 U per gram raw materials, 16 U per gram raw materials and 14 U per gram raw materials respectively, the ratio of raw material to water was 1∶2.5, and the initial pH value of culture mediums was 5.5. Besides, the optimum adding time of the three enzyme was summed up as follows：xylanase and acid protease added before the saccharification and cellu-lase added before the fermentation. Under the above conditions, the output rate of raw materials reached up to 44.54%and the fermentation pe-riod shortened by 12 h.

关 键 词：木聚糖酶 纤维素酶 酸性蛋白酶 玉米原料 酒精发酵 

分 类 号：TQ223.122[化学工程—有机化工]