2013年中国三省贝类及环境中副溶血性弧菌分离株耐药特征分析  

Analysis of antimicrobial resistance results of Vibrio parahaemolyticus isolated from shellfish and its habitat in 2013

作　　者：韩海红[1] 李凤琴[1,2] 徐进[1,2] 陈艳[1,2] 余东敏[1,2] 闫韶飞[1,2] 吴青[1] 李薇薇[1,2] 郭云昌[1,2] 李宁 

机构地区：[1]中国疾病预防控制中心营养与健康所,北京100050 [2]国家食品安全风险评估中心,卫生部食品安全风险评估重点实验室

出　　处：《卫生研究》2015年第3期382-386,共5页Journal of Hygiene Research

基　　金：国家科技支撑计划项目(No.2012BAK01B02)

摘　　要：目的研究四川、福建和广西贝类及其养殖环境中分离的副溶血性弧菌的耐药特征。方法采用微量肉汤稀释法测定186株副溶血性弧菌对8种抗生素包括氨苄西林、头孢噻肟、头孢他啶、庆大霉素、四环素、氯霉素、环丙沙星和复方新诺明的耐药性。结果 186株副溶血性弧菌总耐药率为69.35%,主要耐受的抗生素为氨苄西林,氨苄西林的最小抑菌浓度(MIC)值的几何均数大于耐药解释标准。4株菌表现为多重耐药/中介。所有菌株对头孢噻肟、头孢他啶、氯霉素、环丙沙星等4种抗生素100%敏感。按照分离省份、采样环节和样品种类分类分析,副溶血性弧菌分离株的氨苄西林耐药谱3个采样环节中养殖场的耐药率最高为73.68%,3个省份中四川菌株的耐药率最高为70.94%,但差异均没有统计学意义。58份样品中,有44份样品分离出2株及以上菌株,样品分离菌株的耐药谱多态率为77.27%(34/44)。结论贝类及其养殖环境中副溶血性弧菌的氨苄西林耐药率较高,养殖环境可能是副溶血性弧菌耐药性的主要来源,需加强贝类养殖过程的副溶血性弧菌耐药性的监测和控制。Objective To evaluate the antimicrobial resistance of Vibrio parahaemolyticus isolated from shellfish and its habitat in Sichuan, Fujian and Guangxi. Methods The susceptibility of 186 isolates of Vibrio parahaemolytieus to 8 antibiotics were determined by broth microdilution susceptibility test. The antibiotics of ampicillin, cefotaxime, eefiazidime, gentamicin, tetracycline, chloramphenicol, ciprofloxacin and trimethoprim/sulfamethoxazole were used. Results The antibiotic resistance rate were 69.35% in which ampicillin resistance was the most prevalent. The geometric mean of ampicillin MIC value of all isolates was greater than the interpretation value of resistance. Among the 186 tested isolates, the multiple antibiotic resistance and/or intermediate resistance was 4. All strains were 100% sensitive to cefotaxime, ceftazidime, chloramphenicol and ciprofloxacin. The ampicillin susceptibility spectrum of aquaculture farm was the highest among the three sectors as 73.68% and Sichuan was the highest among the three provinces as 70. 94% although there were no significant differences. There were 44 samples out of which 2 and above strains were isolated, and the susceptibility spectrum polymorphism rate of strains isolated from the same sample was 77.27% （34/44）. Conclusion The ampicillin resistance rate is relatively high, and shellfish habitat may be the main source of antimicrobial resistance of Vibrio parahaemolyticus. There is an urgent need to strengthen the surveillance and management of Vibrio parahaemolyticus in the shellfish breeding process.

关 键 词：贝类 副溶血性弧菌 药物敏感性 微量肉汤稀释法 最小抑菌浓度 

分 类 号：R155.51[医药卫生—营养与食品卫生学]