木醋杆菌合成细菌纤维素增效因子的筛选  

Screening of synergistic factor in the process of bacterial cellulose fermentation with Acetobacter xylinus

作　　者：常冬妹[1,2] 卢红梅[1,2] 陈莉[1,2] 张丽平[1,2] 杨凤仪[1,2] 贾青慧[1,3] 

机构地区：[1]贵州大学贵州省发酵工程与生物制药重点实验室,贵州贵阳550025 [2]贵州大学酿酒与食品工程学院,贵州贵阳550025 [3]贵州大学化学与化工学院,贵州贵阳550025

出　　处：《中国酿造》2015年第5期35-39,共5页China Brewing

基　　金：国家自然科学基金(31160338);贵州省科学技术基金(黔科合J字[2010]2066)

摘　　要：研究了椰子水、玉米浆、Tween-80、羟甲基纤维素(CMC)、烟酸、生物素、咖啡因和海藻酸钠8种物质对木醋杆菌(Acetobacter xylinum)合成细菌纤维素的作用。结果表明,椰子水、玉米浆、Tween-80、CMC、烟酸和生物素对木醋杆菌合成细菌纤维素有促进作用,最适添加量分别为60%、10%、0.8 g/L、4 g/L、1.0 mg/L、25 mg/L,在最适添加量下细菌纤维素产量分别是6.008 g/L、8.534 g/L、2.256 g/L、2.044 g/L、2.842 g/L、3.118 g/L,比起产量为1.506 g/L的空白组有显著增效效果,而其增效作用从大到小依次为玉米浆、椰子水、生物素、烟酸、Tween-80、CMC,海藻酸钠的增效效果不明显,而咖啡因有一定的抑制作用。The effect of coconut water, corn steep liquor, Tween-80, CMC, niacin, biotin, caffeine and sodium alginate addition on the bacterial cellulose fermentation with Acetobacter xylinus was studied. The results showed that coconut water, corn steep liquor, Tween-80, CMC, niacin, and biotin could promote bacterial cellulose yield, and the optimal addition was 60%, 10%, 0.8 g/L, 4 g/L, 1.0 mg/L, 25 mg/L, respectively, and the yield of bacterial cellulose were 6.008 g/L, 8.534 g/L, 2.256 g/L, 2.044 g/L, 2.842 g/L, 3.118 g/L, respectively. Compared with the blank group output （1.506 g/L）, these synergistic effects were significant. The synergistic effect from high to low in order was corn steep liquor, coconut water, biotin, niacin, Tween-80, CMC. The effect of sodium alginate was not obvious, while caffeine had certain inhibitory effect.

关 键 词：木醋杆菌 细菌纤维素 增效因子 筛选 

分 类 号：Q815[生物学—生物工程]