超高效液相色谱-三重四级杆串联质谱仪(UPLC-MS/MS)同时测定白酒中4种微量甜味剂  

Simultaneous Detection of 4 Kinds of Trace Sweeteners in Baijiu(Liquor) by Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry(UPLC-MS/MS)

作　　者：刘国英[1] 胡艳丽[1] 万春环 汤有宏 王银辉[1] 金先锋[1] 

机构地区：[1]安徽古井贡酒股份有限公司,安徽亳州236820

出　　处：《酿酒科技》2015年第6期92-94,共3页Liquor-Making Science & Technology

摘　　要：对检测白酒中微量甜味剂（安赛蜜、糖精钠、甜蜜素、阿斯巴甜），建立了一种利用超高效液相色谱-三重四级杆串联质谱仪（ESI离子源）的检测方法。样品经酒精前处理后，以BEH C18色谱柱为分离柱，0.1%（v/v）乙酸-1 mmol/L乙酸铵溶液∶乙腈为流动相进行梯度洗脱，经超高效液相色谱（UPLC）分离，采用电喷雾串联四级杆质谱进行多反应监测模式（MRM）进行检测。4种甜味剂线性范围在50～250 ng/mL内，拟合所得线性相关系数均大于0.997。在3个加标水平下，得到样品平均回收率为97.1%～111.3%，相对标准偏差（RSD）均控制在5%以内。该方法简单快捷、准确可靠，可用于白酒中4种甜味剂的同时检测。In order to detect the trace sweeteners in Baijiu（liquor） （acesulfame potassium, soluble saccharin, sodium cyclamate and aspartame）, a method using UPLC-MS/MS had been developed in this study. Liquor samples were pre-treated by alcohol, then BEH C18 chromatographic column was used as separation column, and 0.1 % （v/v） acetic acid-1mmol/L ammonium acetate solution/acetonitrile were used as mobile phase for gradient elution. After UPLC separation, MS/MS was adopted for multiple reaction monitoring mode （MRM）. The experimental re-sults suggested that the linear range of standard curves for those 4 sweeteners was from 50 to 250 ng/mL, and the linear relationship was great-er than 0.997. The spiked recoveries were within 97.1%-111.3%in three levels of standard addition among linear range of standard curves. And RSD was controlled below 5%. Such method was simple, quick, accurate and reliable, and it could be used for simultaneous detection of the 4 sweeteners in Baijiu（liquor）.

关 键 词：安赛蜜 糖精钠 甜蜜素 阿斯巴甜 甜味剂 白酒 液质联用仪 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]