冷藏大黄鱼腐败菌群和群体感应信号分子的动态变化规律  

Dynamic Variation Rule of Spoilage Bacterial and Quorum Sensing Signal Molecules in the Refrigerated Large Yellow Croaker

作　　者：朱军莉[1] 赵二科 孙丽霞[1] 黄旭镇 励建荣[2] 田迪英[1] 

机构地区：[1]浙江工商大学食品与生物工程学院,杭州310018 [2]渤海大学省食品安全重点实验室,辽宁锦州121013

出　　处：《中国食品学报》2015年第4期175-182,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31271954);国家科技计划项目(2012BAD29B06);浙江省自然科学基金(LY15C200001);浙江省公益项目(2013C37080)

摘　　要：为阐明水产品贮藏品质劣变与群体感应的内在关联,研究了大黄鱼在4℃冷藏中腐败菌群和群体感应信号分子的动态变化,鉴定和分析了优势腐败菌及其腐败菌产群体感应的能力。结果表明:冷藏大黄鱼细菌生长显著(P<0.05),第5天为货架期终点,其中产H2S菌优势生长,而肠杆菌和乳酸菌生长缓慢。鱼肉贮藏初期检测到AI-2信号分子,其随着细菌增长活性逐步增强(P<0.05),而高丝氨酸内酯类信号分子含量很低。采用16S r DNA和PCR-DDGE技术鉴定冷藏大黄鱼的细菌组成,结果发现在贮藏初期鱼体细菌表现多样性,包括希瓦氏菌(24%)、假单胞菌(24%)、鳗弧菌(24%)等9种细菌;而贮藏终点细菌种类明显下降,希瓦氏菌为优势腐败菌(76.4%),其中波罗的海希瓦氏菌比例最高。进一步发现6株希瓦氏菌都能产生AI-2,其含量存在显著差异(P<0.05),波罗的海希瓦氏菌XH2在对数生长期AI-2活性最高。波罗的海希瓦氏菌是冷藏大黄鱼的优势腐败菌,该菌产生的AI-2信号分子可能参与调控大黄鱼的腐败进程。To evaluate the correlation of quality deterioration and quorum sensing（QS） in aquatic products, the spoilage bacterial and QS signal molecules were determined in large yellow croaker stored at 4 ℃. The main spoilage organism was identified and QS signal molecules produced by isolates were investigated. The results indicated that microbial population in the refrigerated fish increased significantly, and the 5th day was the end of shelf-life. The H2 S producingbacteria were main spoilage organism, while Enterobacteriaceae and lactic acid bacteria increased slowly. The autoinducer-2（AI-2） activity was found in the initial storage of fish matrix, which increased with the growth of bacteria. Additionally, no significant acylated homoserine lactones（AHL） activity was observed. The composition of bacterial flora in the refrigerated large yellow croaker was analyzed based on 16 S rDNA and PCR-DGGE. The nine species of organism were found at 0 day during the refrigerated storage, mainly including Shewanella（24%）, Pseudomonas（24%）, Vibrio anguillarum（24%）, Serratia（7%）. The bacteria species decreased with the storage time, in which Shewanella（76.4%） was colonized by the dominant spoilage organism at 5 day, such as S.baltica. Furthermore, production of AI-2 was observed in six isolates and AI-2 activity differed significantly in each Shewanella strains. S.baltica XH2 produced a pronounced peak of AI-2 towards the exponential growth phase. Thus, S.baltica was the main spoilage organism in the refrigerated large yellow croaker, and AI-2 signal molecule produced by Shewanella might be involved in the regulation of spoilage in the fish during the storage.

关 键 词：大黄鱼 腐败菌 群体感应 AI-2 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]