应用PCR-DGGE对巴氏消毒乳中腐败细菌的污染溯源研究  

Research on pollution sources of spoilage bacteria in pasteurized milk by PCR-DGGE

作　　者：周国君[1,2] 唐玲[3] 胡萍[1] 王晓静 朱建荣 陈韵[1,2] 

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵阳三联乳业有限公司,贵州贵阳550004 [3]遵义医学院,贵州遵义563003

出　　处：《食品工业科技》2015年第16期49-52,57,共5页Science and Technology of Food Industry

基　　金：贵州省科技农业攻关项目[黔科合NY字(2009)3076];贵阳市乳业重大专项[(2009)筑科农合同字2-017-5]

摘　　要：运用PCR-DGGE技术分析了巴氏杀菌乳从原料乳到杀菌、灌装这两个加工关键控制点的微生物动态变化和差异性,容易发生污染的环节是挤乳环节、杀菌后的加工管道和包装环节。原料乳中易携带的细菌是乳酸链球菌(Lactococcus lactis subsp.)和乳酸杆菌(Lactobacillus sp.);挤乳环节中易受无乳链球菌(Streptococcus agalactiae)污染;杀菌后的加工管道易受热杀环丝菌(Brochothrix thermosphacta)、钻黄肠球菌(Enterococcus casseliflavus)、瑞士乳杆菌(Lactobacillus helveticus)和2株非培养的假单胞菌属(Uncultured Pseudomonas sp.)污染;肉杆菌属(Carnobacterium sp.)和产乳酸菌素的肉杆菌(Carnobacterium maltaromaticum)则在挤乳环节、杀菌后的加工管道或包装环节都可能被污染。Abstract;PCR-DGGE technology was applied to analyze the differences and dynamic changes of microbial community of pasteurized milk from key control points of raw milk to pasteurization and filling process. The results showed that the points proned to be contaminated were milk making, processing pipeline after pasteurization and packaging. Lactococcus lactis subsp, and Lactobacillus sp. were easy carried by raw milk. Streptococcus agalactiae was polluted at milk making. Brochothrix thermosphacta, Enterococcus casseliflavus, Lactobacillus helveticus and 2 strains of uncultured Pseudomonas sp. might be polluted at processing pipeline after pasteurization. Carnobacterium sp. and Carnobacterium maltaromaticum may be polluted at milk making, processing pipeline after pasteurization and packaging.

关 键 词：PCR—DGGE 巴氏杀菌乳 腐败微生物 污染溯源 

分 类 号：TS201.1[轻工技术与工程—食品科学]