北京市水产品污染与感染病例中副溶血性弧菌血清型和毒力基因型的比较研究  

Comparative study of serotypes and virulence genes of Vibrio parahaemolyticus isolated from contaminated aquatic products and infection cases in Beijing

作　　者：吴青[1,2] 韩海红[2] 余东敏[2] 胡豫杰[2] 王伟 李志刚[2] 杜春明[2] 徐进[2] 

机构地区：[1]中国疾病预防控制中心营养与健康所,北京100050 [2]国家食品安全风险评估中心 卫生部食品安全风险评估重点实验室,北京100021

出　　处：《中国食品卫生杂志》2015年第4期363-367,共5页Chinese Journal of Food Hygiene

摘　　要：目的对北京市夏季市售水产品污染与感染病例中副溶血性弧菌血清型和毒力基因型进行比较研究,为评估食品安全风险监测的目的与意义提供思路,为北京市水产品副溶血性弧菌污染与临床感染病例的关联性研究提供技术支持。方法对采集的水产品样品和哨点医院腹泻患者粪便样本进行副溶血性弧菌的分离鉴定,采用血清玻片凝集法对分离出的副溶血性弧菌进行血清分型,PCR方法检测菌株的tlh、tdh、trh基因。结果 2014年7~9月共采集水产品样品164份,检出副溶血性弧菌80份,总污染率为48.78%;其中淡水产品污染率为38.78%(19/49),平均菌量浓度为66.63 MPN/g;海水产品污染率为53.04%(61/115),平均菌量浓度为38.14 MPN/g。80株副溶血性弧菌分属于9个血清群,其中O2群28株,占35.00%,O1群11株,占13.75%,O5群10株,占12.50%。80株菌tlh基因均为阳性,只有1株菌携带tdh毒力基因,所有菌株trh毒力基因均为阴性。哨点医院腹泻病人粪便样本中分离鉴定副溶血性弧菌21株,血清型O3∶K6占61.90%(13/21),O4:K8占28.57%(6/21);毒力基因型tdh(+)/trh(-)占95.24%(20/21),tdh(-)/trh(-)占4.76%(1/21)。结论来源于食品样品的副溶血性弧菌绝大部分不具备致病性,而导致消费者腹泻的副溶血性弧菌绝大部分携带致病性毒力基因,表明目前的食品安全风险监测结果不能作为评估副溶血弧菌导致的食源性疾病暴发和散发的依据。Objective The comparative study of serotypes and virulence genes of Vibrio parahaemolyticus isolated from contaminated aquatic products and infection cases in Beijing not only provided clues for the assessment of food safety risk monitoring, but also offered technical support on the correlation research of aquatic products contaminated by Vibrio parahaemolyticus and clinical infections. Methods Vibrio parahaemolyticus isolates were separated from aquatic product and stool samples of patients with diarrhea. Serotypes were tested by serum agglutination, tlh, tdh and trh gene of the strains were detected by PCR. Results 164 aquatic product samples were collected from July to September. Vibrio parahaemolyticus was isolated from 80 aquatic product samples. Total incidence of Vibrio parahaemolyticus was 48.78%. The incidence of Vibrio parahaemolyticus in sea products was 53.04% , and the incidence of V^brio parahaemolyticus in freshwater products was 38.78%. The average concentration in sea products was 38.14 MPN/g, and was 66. 63 MPN/g in freshwater products. 80 isolates were divided into 90 serogroups, among which 02 （35.00%） , Ol （ 13.75% ） and 05 （12. 5% ） were the main serotypes. All 80 isolates had tlh gene, only 1 isolate had tdh gene, and no trh gene was detected. From stool samples of sentinel hospital patients with diarrhea, 21 Vibrio parahaemolyticus strains were isolated and identified. Among 21 clinical strains, 13 strains were 03 ： K6 serotype, and 6 strains were 04 ： K8 serotype, with the ratio of 61.90% and 28.57% respectively. Virulence genotype tdh （ ＋ ） /trh （-） had 20 strains, tdh （-） /trh （-）had 1 strain, with the ratio of 95.24% and 4. 76% respectively. Conclusion China＇s food safety risk monitoring was mainly to evaluate the occurrence of foodborne illness through monitoring in Vibrio parahaemolyticus in aquatic products. Most of the Vibrio parahaemolyticus strains isolated from aquatic product samples were not pathogenic, but all the Vibrio parahaemolyticus wh

关 键 词：水产品 腹泻病例 副溶血性弧菌 血清型 tlh TDH TRH 毒力基因 致病性 风险监测 食源性致病菌 

分 类 号：R155.5[医药卫生—营养与食品卫生学] F762.6[医药卫生—公共卫生与预防医学]