火棘果不同极性组分多酚对油脂的抗氧化活性分析  

Lipid- antioxidant activity of different solvent extracted polyphenols from Pyracantha fortuneana fruit

作　　者：王晓静[1] 陈莉华[1] 徐丹[1] 向明芳[1] 李贤毅[1] 

机构地区：[1]吉首大学化学化工学院,湖南吉首416000

出　　处：《中国油脂》2015年第B10期78-83,共6页China Oils and Fats

基　　金：科技部科技型中小企业技术创新基金资助项目（10C26214302421,11C26214305373）;吉首大学武陵山区特色植物资源开发与应用湖南省研究生培养创新基地开放项目（2014KFXM01）;湖南省科技厅科技计划资助项目（2013FJ3026）

摘　　要：以超声波辅助乙醇提取火棘果多酚，并用石油醚、乙酸乙酯、正丁醇及水顺序对总多酚提取物（PFFP）进行萃取，分别得到4种提取物PFFP1、PFFP2、PFFP3、PFFP4，以没食子酸为对照品测定其多酚含量，考察了提取物对6种油脂的抗氧化效果及抗水解酸败作用并与Ve作比较。结果表明：在实验条件下，1．00g／L的PFFP、PFFP1、PFFP2、PFFP3及PFFP4中多酚含量分别为0．556、0．359、0．542、0．529g／L和0．429g／L。当多酚质量浓度均为0．3g／L时，保护率位列前三的多酚及油脂分别是PFFP4（菜籽油）、PFFP（菜籽油）、PFFP（橄榄油），其保护率分别为86．6％、83．9％、82．8％，抗水解酸败能力位列前三的多酚及油脂分别是PFFP4（菜籽油）、PFFP（菜籽油）、PFFP（橄榄油），其酸值（NaOH）降低值分别为0．96、0．88、0．83mg／g。结果显示不同极性组分的多酚对不同种类油脂的抗氧化能力有差异。The total polyphenol （PFFP） was extracted from Pyracantha fortuneana fruit with ultrasound - assisted ethanol and separated succesively using petroleum ether, ethyl acetate, n - butanol and water, then PFFP1, PFFP2, PFFP3 and PFFP4 were obtained respectively. The polyphenols contents of the ex- tracts were determined using gallic acid as control substance. Antioxidant activities of the extracts were e- valuated using the protection effect on oxidation and the inhibitory effect on rancidity of oils and fats, and compared with VE. The results showed that, under the experimental condition, the polyphenols content of PFFP, PFFP1, PFFP2, PFFP3 and PFFP4 in 1.00 g/L were 0.556, 0.359, 0.642, 0. 529 g/L and 0.429 g/L, respectively. When the polyphenols concentrations were 0.3 g/L, the top three for oxidation protec- tion rates were PFFP4 （86.6% for rapeseed oil）, PFFP（83.9% for rapeseed oil） and PFFP（82.8% for olive oil）, respectively. The top three for acid value reduction was PFFP4 （0.96 mgNaOH/g for rapeseed oil）, PFFP（O. 88 mgNaOH/g for rapeseed oil） and PFFP（O. 83 mgNaOH/g for olive oil）, respectively. The results indicated that antioxidant activities of different solvent extracted polyphenols were different for various oils and fats.

关 键 词：火棘果 多酚 油脂 抗氧化活性 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程] TS202.3[轻工技术与工程—食品科学与工程]