金鲳鱼内脏复合蛋白酶的分离及酶学性质研究  

Isolation of golden pompano visceral proteinases and enzymatic properties

作　　者：肖鑫鑫[1] 申铉日[1] 张培[1] 

机构地区：[1]海南大学食品学院,海口570228

出　　处：《食品科技》2015年第9期140-145,共6页Food Science and Technology

基　　金：十二五国家科技支撑计划项目(2012BAD28B06);海口市重点科技计划项目(2012-053);海南省产学研一体化专项资金项目(CXY20130022)

摘　　要：鱼在加工过程中,内脏一般都是被当成废弃物处理。这样不仅污染环境,还造成了巨大的浪费,综合开发利用鱼类内脏对提高鱼类的商业价值有着重要的意义。以金鲳鱼内脏为原料,采用丙酮除脂、pH7.5的Tris-HCl缓冲溶液匀浆法从中浸提出粗蛋白酶,并依次通过55%的硫酸铵沉淀、透析、Sephadex G-100凝胶等方法对粗蛋白酶进行纯化。探讨了pH、温度、离子等因素对蛋白酶活性的影响,利用紫外分光光度法、Lowry法分别测定酶的活性和蛋白的含量,并对将分离得到的混合蛋白酶和胃蛋白酶活性进行了比较,为金鲳鱼加工废弃物的开发利用奠定了理论基础。实验结果表明,该酶的最适pH值为4.0,为酸性蛋白酶,最适合的温度为25℃,温度范围比较温和,Zn2+、Mg2+等离子对酶的活性有激活作用,而Pb2+、EDTA等对酶活性有抑制作用,酶的活力为64 U/mg,对明胶的水解效果比胃蛋白酶明显。In the process, fish organs are usually treated as garbage, it is not only pollute the environment but also caused great waste, so comprehensive utilization of fish offal has important meaning for the development of commercial fishes. This article takes golden pompano viscera as raw material, using acetone got rid of the fat and then cut it into pieces, digestion by Tris-HCl（pH7.5）, after the centrifugal obtain the clear liquid. Then precipitation by 55% ammonium sulfate, dialysis, Sephadex G-100 gel for purfying the crude protease. Discussion the effects of pH, temperature, ion and other factors on the protease. By UV spectrophotometry measured enzyme activity, Lowry method measured the protein content. The complex protease was compared to Pepsin, theoretical foundation is laid for the development and utilization of marine fishery waste. The experimental results shows that, The optimum pH is 4.0 so this enzyme is acidic protease. The most suitable temperature was 25 ℃. Zn2＋, Mg2＋ and other ions activate the activity of this enzyme but Pb2＋, EDTA inhibited the activity of this enzyme. The activity of enzyme is 64 U/mg, hydrolysis of gelatin obvious than pepsin.

关 键 词：金鲳鱼 内脏 复合蛋白酶 酶学特性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]