响应面法优化鳕鱼皮酸溶性胶原蛋白的提取工艺  

Optimization of extraction condition of acid-soluble collagen from cod skin by response surface methodology

作　　者：淑英[1] 敖冉[1] 宋佳[1] 任惠[1,2] 丁娜[1] 戎平[1] 张志胜[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001 [2]河北省张家口市桥东区姚家庄镇政府,河北张家口075000

出　　处：《食品工业科技》2015年第24期269-272,277,共5页Science and Technology of Food Industry

基　　金：国家海洋公益性行业科研专项经费(201205031)

摘　　要：为了提高鳕鱼皮中酸溶性胶原蛋白的提取率,选取提取液浓度、提取时间和料液比为影响因素,以胶原蛋白提取率为响应值,在单因素实验基础上,根据中心组合(Box-Behnken)实验设计原理,对鳕鱼皮酸溶性胶原蛋白的提取工艺进行优化。结果表明酸法提取胶原蛋白最佳工艺条件:醋酸浓度为0.47 mol/L、料液比为1∶33、提取时间为90.2 h。在此条件下提取所得酸溶性胶原蛋白提取率的理论值为37.92%,实验验证值为37.36%。相对误差为1.49%,说明采用响应面法优化得到的酸法提取鳕鱼皮胶原蛋白的工艺参数准确可靠,为鳕鱼皮酸溶性胶原蛋白的提取提供了一定的技术参考。To improve the extract rate of acid-soluble collagen（ASC） from cod skin,the acid concentration,extraction time and the liquid/material ratio were applied to accelerate the extraction process. With the extract rate of ASC as the response value,based on the single factor experiments,the factors influcing the extraction of ASC were optimized by response surface methodology according to the Box- Behnken central composite designs. The results showed that the acid contraction of 0.47 mol/L,liquid/material ratio of 1∶33（m∶v） and the extract time of 90. 2 h, the predicted extract rate and the actual extract rate were 37. 92 % and 37. 36 %respectively,the deviation was1.49%. The optimized of the factors influcing the extraction of ASC by response analysis methods was accurate and reliable,which gave a technical reference for the extraction of ASC from cod skin.

关 键 词：鳕鱼皮 胶原蛋白 酸法提取 响应面法 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]