不同降解方式对硫酸软骨素寡糖抗氧化活性的影响  

Effects of Different Degradation Methods on Chondroitin Sulfate Oligosaccharide's Antioxidant Activity

作　　者：王俊[1] 曾凡新[2] 程薇[1] 熊光权[1] 乔宇[1] 吴文锦[1] 张金木[1] 鉏晓艳[1] 史德芳[1] 汪兰[1] 

机构地区：[1]湖北省农业科学院农产品加工与核农技术研究所/湖北省农业科技创新中心农产品加工分中心,武汉430064 [2]江西农业大学食品科学与工程学院,南昌330045

出　　处：《湖北农业科学》2015年第22期5707-5710,共4页Hubei Agricultural Sciences

基　　金：湖北省农业科学院青年科学基金项目(2014NKYJJ07);湖北省重大科技创新计划项目(2015ABA038);湖北省科技支撑计划项目(2014BBA158)

摘　　要：以鲟鱼(Acipenser sinensis)硫酸软骨素为原料,分别采用盐酸酸解法、过氧化氢氧化法、草酸降解法和辐照降解法对其进行处理,使得鲟鱼硫酸软骨素的相对分子质量从35 193分别降至1 841、1 155、2 089和3 049,相对分子质量明显减小,且对DPPH·和·OH的清除能力及Fe3+还原能力明显提高。结果显示,过氧化氢降解所得硫酸软骨素寡糖抗氧化性能很好,当浓度为500μg/m L时,对DPPH·和·OH的清除率可达85%和70%。Taking sturgeon chondroitin sulfate as raw materials and adopting HC1 acid solution, hydrogen peroxide, oxalic acid and radiation to degrade it. The results showed that the relative molecular weight of chondroitin sulfate had respectively dropped from 35 193 to 1 841, 1 155, 2 089 and 3 049. Molecular weight was greatly reduced compared with that of the raw material. In addition, the scavenging activity on DPPH o and OH o and the reducing activity on Fe3＋ has been greatly increased. The results showed that chondroitin sulfate with hydrogen peroxide degradation had good oxidation resistance, when the concentration of oligosaccharide is 0.5 mg/mL, the inhibition rate on DPPH. and OH could reach 85% and 70%.

关 键 词：鲟鱼(Acipenser sinensis) 硫酸软骨素 相对分子质量 抗氧化活性 

分 类 号：S98[农业科学—捕捞与储运] TS254[农业科学—水产科学]