咖啡豆α-半乳糖苷酶的同源建模及分析  

Analysis and homology modeling of coffee- bean α- galactosidase

作　　者：陶佳妮[1] 李赟[1] 姚娣[1] 沈汪洋[1,2] 陈轩[1] 周坚[1,2] 

机构地区：[1]武汉轻工大学食品科学与工程学院,湖北武汉430023 [2]农产品加工湖北省协同创新中心,湖北武汉430023

出　　处：《食品工业科技》2016年第1期326-330,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金青年科学基金项目(31301415)

摘　　要：为进一步了解咖啡豆α-半乳糖苷酶的结构,对咖啡豆α-半乳糖苷酶的理化性质、二级结构及三级结构等进行了理论分析和预测,并在三级结构的基础上进行同源建模研究。分析表明,该酶呈酸性,亲水,稳定。二级结构以α-螺旋和无规则卷曲为主,其空间结构与大米α-半乳糖苷酶相似度较高,以此为模版构建了可靠的三维结构模型。模型的一侧是由8个α-螺旋和8个β-转角形成的球状结构,另一侧是8个β-转角。实验结果为咖啡豆α-半乳糖苷酶的空间结构和催化活性位点的研究提供了理论依据。The primary and spatial structures of coffee- bean α- galactosidase were studied to further determine its structure and function. The results showed that the enzyme protein was hydrophilic,acidic and stable. The secondary structure was dominated by α- helices and random coils. The three- dimensional structures of coffeebean α- galactosidase were constructed based on the template of rice α- galactosidase by homology modeling because their spatial structures were similarity.The side of the structure model was 8 α- helices and 8 β- turns,and the other was 8 β- turns. The experimental results provided the theoretical basis for the study on the spatial structure and catalytic site of coffee- bean α- galactosidase.

关 键 词：咖啡豆α-半乳糖苷酶 蛋白质结构分析 同源建模 

分 类 号：TS201.7[轻工技术与工程—食品科学]