肠膜明串珠菌产右旋糖酐蔗糖酶条件优化  

Optimization of producing enzyme conditions for dextransucraseproducing Leuconostoc mesenteroides

作　　者：侯殿志 王晓[1] 朱延光[1] 相萍萍 蓝尉冰[1] 陈华磊[1] 于玥[1] 陈山[1] 

机构地区：[1]广西大学糖业工程技术研究中心,糖业及综合利用教育部工程研究中心,南宁530004

出　　处：《食品科技》2016年第4期2-6,共5页Food Science and Technology

基　　金：国家自然科学基金项目(21264003);2012年广西自然科学基金项目(2012GXNSFAA053029);2013年广西自然科学基金项目(2013GXNSFAA019036)

摘　　要：右旋糖酐蔗糖酶是一种以蔗糖为底物,催化转移D-葡萄糖基生成α-葡聚糖或低聚糖的葡萄糖基转移酶。对肠膜明串珠菌的产酶发酵条件进行了优化,通过正交实验得出该菌的最优产酶条件为蔗糖5 g/100 mL、牛肉膏2 g/100 mL、酵母膏1.25 g/100 mL、pH7.0。采用多级中空纤维超滤膜对粗酶液进行浓缩,使粗酶液酶活由2.31 U/mL提高到了8.78 U/mL,为酶法定向合成右旋糖酐奠定基础。The dextransucrase is a kind of glucosyltransferases that can catalyze the transfer of D-glucopyranosyl units by synthesizing sucrose to α-glucans or oligosaccharides.In this paper,the optimum fermentation conditions of dextransucrase-producing Leuconostoc mesenteroides were researched.Results were concluded that the optimum fermentation conditions were as follows： sucrose concentration was 5 g/100 mL,beef extract concentration was 2 g/100 mL,yeast concentration was 1.25 g/100 mL,pH value was 7.0.Meanwhile,the crude enzyme liquid was concentrated by the multistage hollow fiber ultrafiltration membrane and the crude enzyme liquid enzyme activity increased from 2.31 U/mL to 8.78 U/mL,which laid the foundation for the stereospecific synthesis of dextran.

关 键 词：右旋糖酐蔗糖酶 肠膜状明串珠菌 发酵 正交优化 浓缩 

分 类 号：TS207.4[轻工技术与工程—食品科学]