响应面法优化火麻蛋白提取工艺研究  

Optimization of extraction of hemp protein by response surface methodology

作　　者：卫萍[1,2] 游向荣[1,2] 张雅媛[1,2] 孙健[1,2] 谢小强[1] 李志春[1,2] 李明娟[1,2] 盛金凤[1,2] 刘国明[1,2] 

机构地区：[1]广西农业科学院农产品加工研究所,南宁530007 [2]广西作物遗传改良生物技术重点开放实验室,南宁530007

出　　处：《中国油脂》2016年第5期24-29,共6页China Oils and Fats

基　　金：国家自然科学基金项目(31560437);公益性行业(农业)科研专项(201503001-6);广西自然科学基金项目(2013GXNSFAA019104;2014GXNSFBA118137);广西科学研究与技术开发计划项目(桂科合14123001-9);广西农业科学院基本科研业务专项(2015JZ76;2015YT87;2014YZ34)

摘　　要：以火麻籽为原料,对碱提酸沉法提取火麻蛋白工艺参数进行研究,在单因素试验的基础上,采用响应面分析法对提取温度、提取时间、提取pH和料液比进行优化并得到回归模型。确定的碱提酸沉法提取火麻蛋白最佳工艺参数为:提取温度60℃,提取时间1 h,提取pH 10,料液比1∶9。在最佳工艺条件下,火麻蛋白的提取率为63.5%。回归模型的预测值与实测值的相对误差为2.3%,该回归方程与实际情况拟合较好。DSC分析得出火麻蛋白的变性温度为83.0℃,纯化后的火麻蛋白相对分子质量分布均小于40 k Da。With hemp seed as raw material, the process parameters of alkali extraction and acid precipita- tion of hemp protein were studied. On the basis of single factor experiment, the extraction temperature, extraction time, extraction pH and ratio of material to liquid were optimized by response surface methodolo- gy, and the regression model was finally established. The results showed that the optimal parameters of alkali extraction and acid precipitation of hemp protein were obtained as follows：extraction temperature 60℃ ,extraction time 1 h ,extraction pH 10 and ratio of material to liquid 1：9. Under the optimal conditions, the extraction rate of hemp protein was 63.5%. The relative error between predictive value and experimental value of the regression model was 2.3 %, indicating a good fit between regression equation and actual situation. The DSC analysis results showed that the denaturation temperature of hemp protein was 83.0℃, and the relative molecular weight distributions of purified hemp protein were less than 40 kDa.

关 键 词：火麻籽 火麻蛋白 碱提酸沉 响应面设计 工艺参数 

分 类 号：TQ936[化学工程]