α-环糊精对α-半乳糖苷酶的抑制  

Inhibition of α-Cyclodextrin on α-Galactosidase

作　　者：姚娣[1] 邹伟[1] 陈轩[1] 孙威[1] 李芳[1,2] 沈汪洋[1,2] 

机构地区：[1]武汉轻工大学食品科学与工程学院,武汉430023 [2]农产品加工湖北省协同创新中心,武汉430023

出　　处：《食品工业》2016年第5期124-127,共4页The Food Industry

基　　金：国家自然科学基金青年基金(31301415)

摘　　要：通过单因素试验和正交试验,以相对酶活为考察指标,研究α-环糊精对α-半乳糖苷酶的抑制作用。得出最具抑制效果的反应参数,即在反应体系中,α-环糊精浓度为10 mmol/L,反应时间为90 min,温度为35℃,p H为6.5,在此反应参数条件下,α-半乳糖苷酶抑制作用明显,相对酶活为64%。The inhibition of α-Cyclodextrin on α-Galactosidase was studied by the single factor and orthogonal experiments and the relative activity was the evaluation index. The optimum parameters of α-CD inhibiting α-galactosidase activity were obtained that the α-CD concentration of 10 mmol/L, the reaction time of 90 min, the temperature of 35 ℃, and the p H was 6.5. At this optimum parameters, the relative activity was 64%.

关 键 词：Α-环糊精 Α-半乳糖苷酶 抑制 

分 类 号：TS201.25[轻工技术与工程—食品科学]