常压室温等离子体快速诱变选育单宁酶高产黑曲霉菌株  

Screening of mutants of higher tannase activity strain of Aspergillus niger by atmospheric and room temperature plasmas

作　　者：周罗娜 吴鑫颖[1] 邱树毅[1,2] 熊进[1,2] 任佳明 

机构地区：[1]贵州大学,贵州省发酵工程与生物制药重点实验室,贵阳550025 [2]贵州大学酿酒与食品工程学院,贵阳550025

出　　处：《食品科技》2016年第7期14-19,共6页Food Science and Technology

基　　金：国家高技术研究发展计划“863计划”项目(2014AA021802-3)

摘　　要：利用常压室温等离子体快速诱变技术对产单宁酶黑曲霉菌株B0201进行诱变,通过溴酚蓝平板变色圈法初筛,液态发酵产酶测定法复筛获得突变株B1401,其诱变后的菌株在液态发酵中获得17.61 U/g酶活,提高2倍;并在固态发酵中进行验证,在固态培养中获得酶活27.68 U/g,提高1.64倍。通过因素优化,得出其液态培养产酶的条件为单宁酸浓度5%,葡萄糖浓度2.5%,最适培养时间3.5 d,最适产酶生长温度28℃。In order to obtain an industrial strain with higher tannase activity, the original strain of Aspergillus niger B0201 was mutated by atmospheric and room temperature plasmas. The methods used to screen the mutant strain with higher tannase activity is tablet hydrolysis using bromophenol blue circle method with liquid fermentation enzyme assay got the mutant strain B1401. The strain B1401 enzyme activity in the liquid fermentation is 17.61 U/g, 2 times than original strain. The enzyme activity in the solid state fermentation is 27.68 U/g, 1.64 times than original strain. In the liquid fermentation, the mutant tannic acid concentration optimal enzyme production was 5%, glucose concentration was 2.5%, optimal incubation time 3.5 d, optimum growth temperature for enzyme production was 28 ℃.

关 键 词：常压室温等离子体诱变 单宁酶 黑曲霉 酶活力 

分 类 号：TS201.3[轻工技术与工程—食品科学]