利用大西洋鲑内源酶酶解加工废弃物制取抗氧化肽的研究  

Study on the optimal conditions for preparing antioxidant peptides from Atlantic salmon processing wastes using its endogenous proteases

作　　者：陈琳[1] 李秉钧[1] 冯俊荣[1] 

机构地区：[1]烟台大学海洋学院,山东烟台264005

出　　处：《渔业现代化》2016年第4期64-69,共6页Fishery Modernization

基　　金：山东省科技发展计划项目(2012GHY11515)

摘　　要：大西洋鲑(Salmo salar)加工废弃物中含有丰富的蛋白质,具有较高的开发利用价值,直接遗弃会造成资源浪费和环境污染,探索利用自身内源蛋白酶酶解制取抗氧化肽具有重要的意义。对大西洋鲑内脏所含内源蛋白酶的性质进行了初步研究,测得主要含有3种内源蛋白酶,最适p H为2.0、6.0、9.0,p H 9.0的碱性蛋白酶具有最高的酶比活力。以水解度和总抗氧化活性为指标,通过单因素试验确定大西洋鲑内源蛋白酶处理鲑鱼的加工废弃物干粉料的最适条件,再用正交实验L16(45)对其酶解条件进行优化,确定影响酶解液总抗氧化活性的主要影响因素(P﹤0.05)为温度、p H和酶解时间,比较后得到最佳酶解条件为:温度35℃、加酶量0.7 m L/50 m L、料液比0.7 g/50 m L、p H 9.0、酶解时间7 h,该条件下酶解液的总抗氧化活性为258.29 U,大西洋鲑加工废弃物干粉料水解度为15.00%。研究表明,采用大西洋鲑内源蛋白酶酶解鲑鱼加工下脚料并制取抗氧化肽,具有一定的可行性。The processing residues of Atlantic salmon （ Salmo salar） contain abundant proteins and are of high value for further utilization, while abandoning them would be a waste of resources and might cause environmental pollution. Thus it is significant to research the preparation of antioxidant peptides from Atlantic salmon processing residues with its endogenous proteases. A preliminary study of proteases from Atlantic salmon viscera showed that there were mainly 3 types of endogenous proteases which were most active at pH value of 2.0, 6.0, and 9.0 respectively, and the alkaline protease with optimal activity at pH 9.0 had the highest specific activity level. Atlantic salmon processing residues protein were hydrolyzed by endogenous proteases to produce the antioxidative hydrolysates. The degree of hydrolysis and the total antioxidant activity were compared to find the best single factors; among the single factors studied, temperature, initial pH and time had significant effects on the total antioxidant activity of enzymatic hydrolysis solution （ P 〈0.05） , and the optimum hydrolysis conditions were determined by L16（4^5） orthogonal array design as follows ： temperature 35℃ , dosage of enzyme 0.7mL/50mL, material-to-solvent ratio 0.7g/50mL, pH value 9.0, and time 7h , under which the total antioxidant activity and degree of hydrolysis were 258.29U and 15.00%, respectively. 1 n conclusion, it is feasible and practical to prepare antioxidant peptides from Atlantic salmon processing wastes using its endogenous proteases.

关 键 词：大西洋鲑鱼 内源蛋白酶 酶解 抗氧化肽 正交 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]