植物乳杆菌UN-30菌株高产苯乳酸的发酵条件优化  

Optimization of the production of phenyllactic acid by Lactobacillus plantarum UN-30 with response surface methodology

作　　者：李芬[1] 刘晨 梁茜茜[1] 张丽萍[1,3] 

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319 [2]黑龙江飞鹤乳业有限公司,齐齐哈尔161000 [3]国家杂粮工程技术研究中心,大庆163319

出　　处：《中国食品添加剂》2016年第8期135-141,共7页China Food Additives

基　　金：国家自然基金(31471586)

摘　　要：以实验室复合诱变的植物乳杆菌UN-30为出发菌株,采用响应曲面法对植物乳杆菌UN-30产苯乳酸的发酵条件进行了优化。在单因素试验基础上,采用4因素4水平Box-Behnken试验设计优化接种量、发酵温度、摇床转速及装液量,建立发酵条件的二次多项回归方程,预测得出在接种量5.03%,发酵温度32.19℃,摇床转速160.41 r/min及装液量40.57%时,苯乳酸产量最高。经试验验证,预测值与验证试验平均值接近,在最优条件下,应用反相高效液相色谱法检测植物乳杆菌UN-30菌株的苯乳酸含量,测得其苯乳酸产量达到2930 mg/L,与未优化前的712 mg/L相比,产量提高了4.12倍。表明在优化植物乳杆菌UN-30菌株的发酵条件后,使该菌株产苯乳酸的能力得到了提高,为苯乳酸的工业化生产提供优良菌种和基础数据。The mutation of Lactobacillus plantarum UN-30 was the original strain, response surface methodology （RSM） was employed to enhance the production of phenyllactic acid by Lactobacillus plantarum UN-30. four-level Box-Behnken design was employed to optimize the medium composition and culture conditions for the production of the phenyllactic acid. Using this methodology, the quadratic regression model of producing phenyllactic acid was built and the optimal combinations of media constituents and culture conditions for maximum phenyllactic acid production were determined as inoculum amount 5.03%, fermentation temperature 32.19℃, rotation speed 160.41 r/min, and liquid volume 40.57%. Phenyllactic acid production obtained was consistent with the predicted value, and the model was proven to be adequate. Application reversed-phase high performance liquid chromatography detected that phenyllactic acid production was increased to 2930 mg/L, which was 4.12 times higher than that of the originalstrain of 712 mg/L. Through the optimization of fermentation conditions of Lactobacillus plantarum UN-30 strain, phenyllactic acid-producing ability was greatly improved. The experiment provided an excellent strain and technical support for the industrial production of phenyl lactic acid.

关 键 词：植物乳杆菌 苯乳酸 发酵条件 响应面优化 

分 类 号：TS202.3[轻工技术与工程—食品科学] TQ920.6[轻工技术与工程—食品科学与工程]