响应面法优化纤维素酶提取真姬菇SDF工艺研究  

Optimization of the SDF extraction of Hypsizigus marmoreus by cellulase

作　　者：郑守晶[1] 王益晨 杨慧芳[1] 郑明锋[2] 

机构地区：[1]福建农林大学金山学院,福建福州350002 [2]福建农林大学食品科学学院,福建福州350002

出　　处：《武夷科学》2016年第1期119-126,共8页Wuyi Science Journal

摘　　要：以真姬菇(Hypsizigus marmoreus)下脚料为原料,采用纤维素酶提取可溶性膳食纤维(soluble dietary fiber,SDF),在加酶量、酶解温度、酶解时间、酶解p H等单因素试验的基础上,采用响应面法分析优化工艺参数。结果表明,最佳提取工艺:纤维素酶添加量为0.57%、酶解温度为57.98℃、酶解时间为3.70 h、酶解pH为5.15,在此条件下,真姬菇SDF提取率达到10.20%。Cellulase was prepared for extracting the soluble dietary fiber（SDF） from Hypsizigus marmoreus scraps. The technological parameters such as enzymatic amount, enzymolysis temperature, enzymolysis time and enzymolysis pH value were subjected to the single factor test, and optimized by response surface analysis. The results showed that the extraction rate could be up to10.20%, when the enzymatic amount was 0.57%, enzymolysis temperature 57.98 ℃, enzymolysis time 3.70 h and the pH 5.15.

关 键 词：真姬菇 可溶性膳食纤维 纤维素酶 提取 

分 类 号：TS209[轻工技术与工程—食品科学] TQ925[轻工技术与工程—食品科学与工程]