甜叶菊废渣提取物抑菌活性及抑菌稳定性研究  

Antibacterial activity and stability of Stevia rebaudiana waste extract

作　　者：赵磊[1] 林文轩[1] 迟茜[1] 王成涛[1] 徐美利 连运河 

机构地区：[1]北京工商大学食品营养与人类健康北京高精尖创新中心,北京市食品添加剂工程技术研究中心,北京100048 [2]晨光生物科技集团股份有限公司,河北邯郸057250

出　　处：《食品工业科技》2016年第24期168-172,共5页Science and Technology of Food Industry

基　　金：北京市科技计划项目(Z151100001215008);北京市属高校创新能力提升计划项目(0142132016);北京工商大学大学生科学研究与创业行动计划项目(SJ201601031)

摘　　要：研究甜叶菊废渣提取物的抑菌活性及抑菌稳定性。甜叶菊絮凝废渣经酸性丙酮提取和乙酸乙酯萃取后得到甜叶菊废渣提取物。采用滤纸片扩散法和琼脂稀释法等测定甜叶菊废渣提取物的抑菌活性,并探讨热处理、紫外光、p H和金属离子对甜叶菊废渣提取物抑菌活性的影响。结果表明,甜叶菊废渣提取物对金黄色葡萄球菌、蜡样芽孢杆菌和枯草芽孢杆菌具有良好的抑菌作用,最小抑菌浓度(或最小杀菌浓度)分别为3.13(6.25)、12.5(25)和12.5(12.5)mg/m L,但对大肠杆菌和沙门氏菌无抑菌作用。甜叶菊废渣提取物经100℃处理20 min仍具有较好的抑菌活性,紫外线处理使其对蜡样芽孢杆菌的抑菌活性降低,p H>7.0时对蜡样芽孢杆菌无抑菌作用,Na+、K+和Ca2+能够增强其对金黄色葡萄球菌和枯草芽孢杆菌的抑菌活性,Fe2+和Fe3+使其抑菌活性丧失或降低。甜叶菊废渣提取物作为天然防腐剂使用具有广阔的开发和利用前景。The objective of the study was to evaluate the antibacterial activity and stability of Steva rebaudiana waste extract.Stevia rebaudiana waste extract was obtained from flocculent waste of Stevia rebaudiana by acid acetone and ethyl acetate extraction.The antibacterial activity of Stevia rebaudiana waste extract was evaluated by filter paper diffusion and agar dilution method.The effect of temperature, ultraviolet light, pH and metal ion on the antibacterial activity of Stevia rebaudiana waste extract was also studied.The results showed that Stevia rebaudiana waste extract had antibacterial activity against Staphylococcus aureus, Bacillus cereus and Bacillus subtilis, with the minimal inhibitory concentration （ or minimum bactericidal concentration） of 3.13 （6.25）, 12.5 （25）, and 12.5 （12.5） mg/mL, respectively. However, it had no antibacterial activity against Escherichia coil and Samonella typhimurium.Stevia rebaudiana waste extract still showed good antibacterial activity after heating at 100 ~C for 20 min.The antibacterial activity against Bacillus cereus decreased after ultraviolet light treatment, and eliminated at pH 〈 7.0.Na ＋ , K ＋ and Ca2~ increased the antibacterial activity against Staphylococcus aureus and Bacillus cereus, while Fe2＋ and Fe3＋ decreased or deprived its antibacterial activity.In conclusion, Stevia rebaudiana waste extract had a broad prospect of development and utilization by using as a natural preservative.

关 键 词：甜叶菊 废渣 抑菌 食源性细菌 

分 类 号：TS201.1[轻工技术与工程—食品科学]