带鱼蛋白水解液抑制二肽基肽酶IV活性的研究  

Research on Inhibition of DPP-IV by Hydrolysates of Hairtail

作　　者：靳挺[1] 武玉学[1] 刘春平[1] 

机构地区：[1]浙江大学宁波理工学院生物与化学工程学院,浙江宁波315100

出　　处：《中国食品学报》2016年第9期48-52,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：宁波市自然科学基金项目(No.2014A610204);宁波市海洋与渔业局项目(No.2-2);宁波市科技惠民项目(No.2015C50050)

摘　　要：本文首次报道酶解带鱼蛋白获得具有抑制二肽基肽酶IV活性的水解液。探讨Flavourzyme风味蛋白酶水解带鱼蛋白以及水解液抑制二肽基肽酶IV的活性,获得优化的酶解工艺。采用单因素试验研究酶解时间、加酶量、温度和鱼水比对DPP-IV抑制的影响,并在此基础上进行正交试验,得出优化的酶解条件。采用底物发色法测定DPP-IV抑制率,结果表明:在优化的酶解条件下:温度45℃,加酶量1.6%,酶解时间4.5 h,鱼水比1∶3,带鱼蛋白水解液对DPP-IV的抑制率达96.97%。This is the first reported the hydrolysate of hairtail protein inhibited dipeptidyl peptidase IV activity. In order to obtain the optimal process conditions of the enzymatic hydrolysis, the hairtail protein was hydrolyzed by Flavonrzyme and inhibition of hydrolysate on DPP-IV was researched. The factors such as enzymatic hydrolysis time, en- zyme amount and fish-water ratio, enzymatic hydrolysis temperature were researched by single factor experiment and the optimum conditions were obtained by the orthogonal experiments. The inhibition rate of DPP-IV was measured by using the ehromogenic substrate method. The optimal conditions for enzymatic hydrolysis of hairtail protein were as following： 1： 3 of fish-water ratio, the amount of enzyme 1.6%, 4.5 h of hydrolysis time, 45 % of hydrolysis temperature. Under the optimum conditions, the inhibition rate of DPP-IV of the hydrolysate was up to 96.97%.

关 键 词：二肽基肽酶IV 抑制率 带鱼 酶解 风味蛋白酶 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]