Pichia pastoris细胞的酶法水解  

Enzymatic hydrolysis of Pichia pastoris cells

作　　者：张永杰[1] 牛丹丹[1,2] 吴海洋[1] 黄磊[1] 董自星[3] 刘晓光[3] 叶秀云[2] 路福平[1] 

机构地区：[1]天津科技大学生物工程学院,工业发酵微生物教育部重点实验室,天津300457 [2]福州大学生物科学与工程学院,福建省海洋酶工程重点实验室,福建福州350116 [3]天津科技大学化工与材料学院,生物化工系,天津300457

出　　处：《食品与发酵工业》2017年第1期80-84,90,共6页Food and Fermentation Industries

基　　金：天津市高等学校科技发展基金计划项目(20130628);天津市应用基础与前沿技术研究计划(青年基金项目)(14JCQNJC09200);福建省教育厅产学研(JA15049);国家自然科学基金国际(地区)合作与交流项目(31461143026)

摘　　要：以工业酶制剂生产中的废弃毕赤酵母细胞为对象,研究并建立了酶法制备酵母浸出物的新工艺。新工艺为:10%(w/v)废弃毕赤酵母菌体中加入β-甘露聚糖酶、β-葡聚糖酶和果胶酶,于50℃作用12 h;再加入中性蛋白酶,于50℃作用7 h;再加入DNA酶和RNA酶,于37℃作用4 h。离心收集上清液,真空冷冻(或喷雾)干燥获得酵母浸出物。运用这一技术,酵母细胞组分的溶出率达到66.2%,固形物收得率为65.7%。使用该研究制得的酵母浸出物培养重要工业微生物菌种,其支撑细胞生长繁殖的效果显著优于市售酵母粉。Yeast extract contains abundant nutrition and has wide applications in condiment, pharmaceuticals, health care, microbial fermentation and other industries. Using spent yeast cell mass of Pichia pastoris formed during the production of industrial enzymes as raw materials, a novel enzymatic process for preparing yeast extract was estab- lished as follows. Spent cells of P. pastoris were re-suspended to a final concentration of 10% （w/v）. 13-Mannases,-glucanase and pectinase were then added simultaneously to hydrolyze yeast cells at 50 ℃ for 12 h. The hydrolysis was then continuously carried out with neutral protease at 50℃ for 7 h. Additionally, the reaction mixture was treated with DNase and RNase for another 4 h. After all enzymatic treatments had been administered, the hydrolysate was centrifuged, and yeast extract was obtained from the supernatant by vacuum freeze-drying or spray drying, with a dis- solution rate of 66.2% and a dry matter yield of 65.7%. Yeast extract prepared in this study was much better than that from commercial sources in cultivating microorganisms, including industrially important strains Escherichia coli, Bacillus licheniformis, and P. pastoris.

关 键 词：酵母浸出物 酶法制备 毕赤酵母 

分 类 号：TS201.3[轻工技术与工程—食品科学]