常压室温等离子体快速诱变选育高产单宁酶菌株  

The rapid mutant strains of high tannase yield obtained by atmospheric and room temperature plasmas

作　　者：熊进[1,2] 吴鑫颖[2] 邱树毅[2] 简辉[2] 周罗娜 任佳明 

机构地区：[1]广州市微生物研究所,广东广州510663 [2]贵州大学酿酒与食品工程学院,贵州贵阳550025

出　　处：《食品工业科技》2017年第4期225-230,共6页Science and Technology of Food Industry

基　　金：国家高技术研究发展计划(863计划)项目(2014AA021802)

摘　　要：采用常压室温等离子体诱变技术处理黑曲霉B0201,选育高产单宁酶突变株,为单宁酶的发酵生产提供优良菌株。确定等离子体处理条件,采用稀释平板培养法挑选突变株,利用变色圈法和分光光度法测定产酶量,获得最佳诱变时间为180 s,正突变率高达19%。通过2%的高浓度单宁酸显色平板初筛850株菌,液态摇瓶发酵复筛90株菌,得到高产单宁酶的菌株B1401。诱变后单宁酶酶活为17.61 U/g,是原始菌株酶活8.94 U/g的2倍左右,且传代菌种产单宁酶稳定性良好。Atmospheric and room temperature plasmas（ ARTP） was used to induce Aspergillus niger B0201,in order to obtain mutant strains with enhanced ability to produce tannase. The optimal treatment condition were determined,and dilution pate culture method was used to screen mutant strains.The color changing circle method and spectrophotometry was used to analyze tannase production,and the best mutation time was 180 s,and the positive mutation rate was 19%. The high concentration of2% tannic acid was utilized for color plate screening 850 strains.90 strains of bacteria were screened by liquid shake flask fermentation,and the strain of extraordinary yield of B1401 was achieved.After the mutation,the enzyme activity was 17.61 U / g,which was about double as much as that of the original strain 8.94 U / g,and the stability of the enzyme production was good.

关 键 词：单宁酶 黑曲霉 常压室温等离子体 诱变选育 

分 类 号：TS201.3[轻工技术与工程—食品科学]