海南发酵食品鱼茶中乳杆菌发酵特性的研究  

Fermentation characteristics of Lactobacillus isolated in Hainan fermented food Yucha

作　　者：徐传标 霍冬雪[1] 胡淇淞 李从发[1] 张家超[1] 

机构地区：[1]海南大学食品学院,海南海口570228

出　　处：《食品工业科技》2017年第7期117-120,共4页Science and Technology of Food Industry

基　　金：海南省高等学校科学研究项目(Hnky2016-12);海南大学科研启动基金(KYQD1548)

摘　　要：在前期研究中,从海南省传统发酵食品鱼茶中分离获得144株乳杆菌,主要隶属于植物乳杆菌、戊糖片球菌、香肠乳杆菌、短乳杆菌、棒状乳杆菌、鼠李糖乳杆菌及干酪乳杆菌等。本文从发酵应用的角度出发,对分离得到的乳杆菌进行了一系列的鱼肉发酵特性实验,包括p H变化率实验,耐食盐、耐亚硝盐实验、氨基酸脱羧酶实验等。实验结果表明:R19-5,M1-4,R21-3,R21-4和RD-1五株菌具有快速产酸能力,经过12~16 h发酵后,p H分别能达到3.75、3.89、3.86、3.73和3.74,同时都能耐受150 mg/kg Na NO2溶液和8%盐浓度;菌株在发酵时都不产氨基酸脱羧酶。综合实验结果,筛选出具有优良发酵特性的植物乳杆菌(Lactobacillus plantarum)3株,香肠乳杆菌(Lactobacillus farciminis)1株和短乳杆菌(Lactobacillus brevis)1株。本研究将对寻找优良的肉制品发酵剂和合理利用新的生物资源具有一定的理论指导作用。In previous study, a total of 144 strains of Lactobacillus were isolated from Yucha, a traditional fermented food of Hainan province. After identification, these strains mainly belonged to the Lactobacillus plantarum, Lactobacillus pentosus, Lactobacillus farciminis, Lactobacillus brevis, Lactobacillus casei, Lactobacillus coryniformis and LactobaciUus rhamnosus.From the perspective of fermentation application, we had made a series of Lactobacillus fermentation characteristic test, including acidification rate test,resistance to salt and the nitrate test, amino acid decarboxylase test.Experimental resuhs showed that： R19-5, M1-4, R21-3, RD- 1 and R21-4 had fast capacity of acid, after 12- 14 h after fermentation,pH can reach 3.75,3.89, 3.86,3.89 and 3.86, both tolerated concentration of 150 mg/kg NaNO2 and 8% NaCl. Strains in the fermentation did not produce amino acid decarboxylase. The results showed that 3 Lactobacillus plantarum strains, 1 Lactobacillus farciminis strain and 1 Lactobacillus brevis with excellent fermentation characteristics were selected based on the comprehensive test. This research had certain theoretical guidance for looking for excellent meat starter cultures and rational utilization of biological resources.

关 键 词：鱼茶 乳杆菌 发酵性能 

分 类 号：TS201-3[轻工技术与工程—食品科学]