响应面法优化超声波辅助提取鸡枞菌多糖及其抗氧化活性研究  

Optimization of Ultrasound Assisted Extraction of Collybia albuminosa Polysaccharides and Its Antioxidative Activity by Response Surface Method

作　　者：张恒[1] 郑俏然[2] 李敏[2] 

机构地区：[1]雅安职业技术学院药检系,四川雅安625000 [2]长江师范学院生命科学与技术学院,重庆涪陵408100

出　　处：《食品与发酵科技》2017年第2期13-18,121,共7页Food and Fermentation Science & Technology

基　　金：2015年重庆市教委科学技术研究项目(KJ15012018)

摘　　要：本研究采用响应面法优化超声波提取鸡枞菌多糖的工艺条件,并进一步研究了其抗氧化活性。在单因素的基础上,选取超声波作用时间、温度及液固比为影响因子,应用Box-Behnken中心组合进行3因素3水平的实验设计,以多糖提取率为响应值,进行响应面分析。结果表明,超声波提取鸡枞菌多糖的最佳条件为超声波作用时间36min、温度41℃、液固比37∶1时,可得最大多糖提取率,预测值为6.36%,与验证值(6.32%)基本一致。鸡枞菌多糖对羟基自由基(·OH)、超氧阴离子自由基(O_2^-·)及DPPH自由基都有较强的清除能力,具有良好的抗氧化活性。Response surface analysis methodology was used for optimizing the ultrasound extraction process of Collybia albuminosa polysaccharides. Using extraction rate of Collybia albuminosa polysaccharides as the indicator, effects of extraction temperature, time and water-material ratio extraction on the extraction rate of polysaccharide were investigated. Based on single factor studies, a three-variable, three-level Box-Benhnken experimental design and response surface methodology were used to obtain the optimized extraction conditions of polysaccharides. The optimum ultrasound extraction conditions for Collybia albuminosa polysaccharides were extraction time of 36min, extraction temperature of 41℃, and water-material ratio of 37：1 （mL/g）, under which the extraction rate of polysaccharide was up to 6.36%, and the actual extraction rate is 6.32%. Moreover, the scavenging effects of Collybia albuminosa polysaccharides on .OH, O2- and DPPH radical was strong, the results showed that the Collybia albuminosa polysaccharides had good antioxidant activity.

关 键 词：超声波提取 多糖 鸡枞菌 响应面法 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]