基于黑木耳菌Aas1502液态深层发酵培养基组成的优化  

Medium Optimization for Extracellular Polysaccharide Production of Auricularia Auricula strain 1502 by Submerged Fermentation

作　　者：刘俊梅[1] 丁伟[1] 王庆[1] 杨盼盼[1] 王玉华[1] 朴春红[1] 于寒松[1] 李琢伟[2] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]长春职业技术学院,吉林长春130033

出　　处：《食品研究与开发》2017年第5期196-200,215,共6页Food Research and Development

基　　金：吉林省重大科技攻关项目(20140203019NY)

摘　　要：以胞外多糖产量和菌丝体干重为检测指标,优化黑木耳菌Aas1502胞外多糖液态深层发酵培养基组成。通过单因素试验确定培养基碳源和氮源种类及培养基中碳源、氮源、KH_2PO_4和MgSO_4·7H_2O的浓度。利用正交试验进一步优化,并通过方差分析最终确定优化培养基组成为:马铃薯淀粉20g/L、葡萄糖20g/L、蛋白胨4g/L、KH_2PO_4 1.5 g/L、MgSO_4·7H_2O 0.2 g/L,此时胞外多糖产量和菌丝体干重,分别为9.36g/L和12.27 g/L,胞外多糖比优化前提高了1.48倍为后续利用深层发酵提取黑木耳多糖提供了理论基础。The composition of submerged fermentation medium for extracellular polysaccharides from Auricu- laria A uricula strain 1502 was optimized in this study. Production of extracellular polysaccharide and mycelium dry weight were regarded as research index to determine the composition of the medium. Carbon sources, nitro- gen sources, carbon concentration, nitrogen concentration, KH2PO4 concentration and MgSO4·TH2O concentration were determined by single-factor test. Orthogonal test was used to analysis the variance of the two indexes, the optimal composition of culture medium were determined ： potato starch 20 g/L, glucose 20 g/L, peptone 4 g/L, KH2PO4 1.5 g/L and MgSO4· 7H2O 0.2 g/L. Production of extracellutar polysaccharide and mycelium dry weight were 9.36 g/L and 12.27g/L, respectively. The effect was significant that the production of extracellular polysaccharide was 1.48 times as before. A theoretical basis was provided for the subsequent extraction of polysaccharides from A uric ularia A uric ula strain using submerged fermentation by this result.

关 键 词：液态深层发酵 正交优化 胞外多糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]