2014年全国食品安全省级监测点微生物质控考核结果  

Quality control results of microbiology of provincial laboratories in China in 2014

作　　者：韩海红[1] 李凤琴[1] 徐进[1] 白莉[1] 王伟 胡豫杰[1] 李业鹏[1] 裴晓燕[1] 余东敏[1] 

机构地区：[1]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021

出　　处：《卫生研究》2017年第3期429-433,共5页Journal of Hygiene Research

基　　金：卫生行业科研专项(No.201302005);国家食品安全风险评估中心高层次人才发展"523"项目

摘　　要：目的对参加国家食品安全风险监测的32个省级疾病预防控制中心,开展金黄色葡萄球菌定量、致泻性大肠埃希菌和未知肠道致病菌定性的质量控制考核,以评价其微生物检验技术能力。方法制备2种浓度的金黄色葡萄球菌样品。制备4种不同血清型的致泻性大肠埃希菌和未知肠道致病菌样品。金黄色葡萄球菌报告结果计算均值和标准差,利用Z比分做出满意与否评价。致泻性大肠埃希菌和未知肠道致病菌的结果评价为正确或者错误,没有报告结果的评价为缺失。结果金黄色葡萄球菌的满意率为82.81%;致泻性大肠埃希菌的正确率为45.16%;未知肠道致病菌考核中,沙门菌血清型的正确率为90.63%。3项考核均正确的率为28.13%。结论 32个省级疾病预防控制中心具备沙门菌鉴定和血清分型以及金黄色葡萄球菌定量检验能力,致泻性大肠埃希菌的血清分型能力有待加强。Objective To get a baseline of 32 provincial center for disease control and prevention（CDC） microbiology laboratory in the aspect of quantitative test ability of Staphylococcus aureu, qualitative test ability of diarrheagenic Escherichia coli and unknown intestinal pathogen, and to comprehend the quality of microbiology testing. Methods Two different concentration samples of Staphylococcus aureus Ⅰ and Ⅱ were made. Diarrheagenic Escherichia coli and unknown intestinal pathogen samples were transported using the special semi-solid AGAR transport medium, in the form of four pure different serotypes each. The results of Staphylococcus aureu were logarithmic transformed, and evaluated with Z-score method using the average and standard deviation. The reporting results of diarrheagenic Escherichia coli and unknown intestinal pathogen were evaluated as right or wrong, and non-reporting were evaluated as missing. Results The satisfaction rate of Staphylococcus aureus was 82.81%. The accuracy rate of diarrheagenic Escherichia coli was 45.16%. Of the 32 results of unknown intestinal pathogen, 29 CDCs reported correct Salmonella serotypes, and the accuracy rate was 90.63%. Combined the three quality control results, 9 CDCs reached the accurate results, with the accuracy rate of 28. 13%. Conclusion 32 provincial CDCs have the identification ability of Salmonella serotyping and qualitative test ability of Staphylococcus aureu, while they need to strength diarrheagenic Escherichia coli identification and serotyping ability.

关 键 词：金黄色葡萄球菌 致泻性大肠埃希菌 沙门菌 微生物 

分 类 号：R155.51[医药卫生—营养与食品卫生学] TS207.4[医药卫生—公共卫生与预防医学]