短梗五加果多酚预防大鼠动脉粥样硬化作用  

Preventive Effect of Polyphenols Isolated from Acanthopanax sessiliflorus Fruits on Atherosclerosis in Rats

作　　者：何忠梅[1] 李成恩[1] 段翠翠[2] 赵玉娟[2] 高磊[2] 栾畅 张连学[1] 李盛钰[2] HE Zhongmei;LI Cheng’en;DUAN Cuicui;ZHAO Yujuan;GAO Lei;LUAN Chang;ZHANG Lianxue;LI Shengyu(College of Traditional Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China;Institute of Agro-Food Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China)

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]吉林省农业科学院农产品加工研究所,吉林长春130033

出　　处：《食品科学》2018年第1期200-206,共7页Food Science

基　　金：长春市产学研协同创新示范点建设项目(16CX20);国家现代农业(奶牛)产业技术体系建设专项(CARS-36);"十三五"国家重点研发计划重点专项(2016YFC0500300);吉林省中医药产业发展专项(20140311029YY)

摘　　要：目的:研究短梗五加果多酚(Acanthopanax sessiliflorus fruit polyphenols,ASFP)对大鼠动脉粥样硬化(atherosclerosis,As)的预防作用,并探讨其作用机制。方法:40只雄性SD大鼠随机平均分成4组,每组10只。采用高脂饮食结合腹腔注射VD3的方法建立As模型,不同剂量ASFP给药组自造模开始之日起分别灌胃150、75 mg/(kg·d)的ASFP,空白组和高脂饮食组大鼠灌胃等量生理盐水,各实验组连续处理12周后,检测大鼠血清生理生化水平并计算As指数;苏木素-伊红染色观察大鼠主动脉As病变情况;Western blot检测主动脉中黏附分子和丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路关键基因的蛋白表达水平。结果:与高脂饮食组相比,ASFP能显著降低大鼠血清中的血脂、黏附分子和炎症因子水平,减少主动脉中的脂质沉积,改善各层结构排列紊乱,显著降低主动脉中细胞间黏附分子-1、血管细胞黏附分子-1、磷酸化p38和磷酸化细胞外信号调节蛋白激酶1/2(phospho-extracellular signal regulated kinases 1/2,p-ERK1/2)的蛋白表达量。结论:ASFP具有预防As的作用,其作用可能与其降血脂和抑制p38 MAPK和ERK1/2 MAPK信号通路,进而抑制炎症因子的表达有关。Objective： A rat model was used to investigate the preventive effect of Acanthopanax sessiliflorus fruit polyphenols （ASFP） on atherosclerosis （As） and the underlying mechanism was explored. Methods： The atherosclerosis model was built by feeding rats with high-fat diet （HFD） combined with intraperitoneal injection with vitamin D3. Forty male rats were randomly divided into four groups （n = 10）： control, HFD, HFD ＋ low-dose ASFP and HFD ＋ high-dose ASFD groups. The rats from the ASFD-treat groups were daily administered with 150 or 75 mg/（kg·d） ASFP during the HFD feeding period of 12 days, while those from the control and HFD groups were given an equal volume of normal saline. Serum biochemical parameters were detected and atherosclerosis index （AI） was calculated at the end of administration. Hematoxylin-eosin （HE） staining was used to observe the atherosclerosis lesion in the aorta of rats. The expression levels of adhesion molecules in the aorta and key genes involved mitogen-activated protein kinase （MAPK） signaling pathway were measured by Western blot. Results： The levels of blood lipids, adhesion molecules and inflammatory cytokines in rats were significantly decreased by ASFP compared with the model group. In addition, after treatment with ASFP, aortic lipid deposition was reduced and the structure of disordered layers was significantly improved compared with the model group. Furthermore, the expression of intercellular cell adhesion molecule-1 （ICAM-1）, vascular cell adhesion molecule-1 （VCAM-1）, phospho-p38 （p-p38） and phospho-ERK1/2 （p-ERK1/2） in the rat aorta were significantly down-regulated by treatment with ASFP compared with the model group. Conclusion： ASFP plays an important role in preventing atherosclerosis, which may be related to the reduction of blood lipids and the inhibition of p38 MAPK and ERK1/2 MAPK signaling pathways, thereby inhibiting the expression of infiammatory cytokines.

关 键 词：短梗五加果 多酚 动脉粥样硬化 降血脂 抗炎 

分 类 号：TS201.4[轻工技术与工程—食品科学]