固态发酵玉米黄粉饲料复合菌种的筛选  

Screening of mixed strains for preparing corn gluten meal feeds by solid-state fermentation

作　　者：江成英[1,2] 王松 李琰[1,2] 徐婷婷[1,2] 郭宏文 张文学[1,2] 韩国栋[1,2] 姜未公 

机构地区：[1]齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔大学农产品加工黑龙江省普通高校重点实验室,黑龙江齐齐哈尔161006

出　　处：《中国酿造》2018年第2期71-74,共4页China Brewing

基　　金：黑龙江省教育厅基本业务专项(LTSW201708)

摘　　要：运用平板点种法、点种刺激圈法和多菌种固体发酵法3种主要方法,筛选发酵玉米黄粉饲料的复合菌株。结果表明,枯草芽孢杆菌(Bacillus subtilis)、凝结芽孢杆菌(Bacillus coagulans)和地衣芽孢杆菌(Bacillus licheniformis)3种菌株以1∶1∶1的比例混合,按5%的接种量接入发酵培养基,发酵效果较好,于30℃条件下发酵120 h,玉米黄粉饲料中可溶性蛋白含量由3.78%提高到14.26%。The mixed strains for fermented corn gluten meal feed were screened by dot-inoculating, dot-inoculating stimulus circle method and mul-ti-strains solid-state fermentation. The results showed that the three strains of Bacillus subtilis, Bacillus coagulans and Bacillus licheniformis were mixed by 1-1-1 with inoculum 5% in the fermentation medium, the fermentation effect was better. Under the conditions of 30 ^ for fermentation 120 h, the soluble protein content in corn gluten meal feed increased i^om 3.78% to 14.26%.

关 键 词：玉米黄粉 复合菌种 固体发酵 可溶性蛋白 蛋白酶活力 

分 类 号：S816.4[农业科学—饲料科学]