短小芽孢杆菌E-1-1-1中AFM_1降解酶的分离纯化  

Separation and Purification of Extracellular Enzyme to Degrade Aflatoxin M_1 from Bacillus pumilus(E-1-1-1)

作　　者：武瑞霞[1] 翟一潭 霍超[1] 王向红[1] 刘晓宇[1] 桑亚新[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071000 [2]江南大学食品学院,江苏无锡214122

出　　处：《中国食品学报》2017年第10期128-133,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家公益性行业科研专项(201205031);河北省科技支撑项目(12271008D;13273203D)

摘　　要：黄曲霉毒素M_1是黄曲霉毒素B_1的羟基化衍生物,主要存在于乳及乳制品中,对肝脏有致畸和致癌作用。目前人们对黄曲霉毒素M_1污染的关注度很高,解决黄曲霉毒素M_1污染问题越发重要。本文在先前研究的基础上,从短小芽孢杆菌(E-1-1-1)的发酵液中通过离心去菌体,硫酸铵沉淀,透析除盐制得粗酶液,再采用超滤,Sephadex G-75凝胶过滤,DEAE-Sepharose Fast Flow离子交换分离纯化获得一种电泳纯的黄曲霉毒素M_1的降解酶,AFM_1降解率为28.9%,经SDS-PAGE测定其分子质量约为58 ku。研究结果为后期进一步研究该降解酶的酶学性质及其降解机理提供了基础。Aflatoxin M_1 is the hydroxylated metabolite of afaflatoxin B_1 and it can be found in milk and dairy products.Aflatoxin M_1 has teratogenic and carcinogenic effects on humans and animals.The existence of aflatoxin M_1 caused more and more people＇s attention.Solve the problem of aflatoxin M_1 pollution is more and more important.on the identification of AFM_1 that is degraded by some secretions from Bacillus pumilus（E-1-1-1） and understand the degradation mechanism is by some extracellular enzymes of Bacillus pumilus（E-1-1-1）.In this paper,on the basis of previous research the fermented liquit medium by Bacillus pumilus（E-1-1-1） was centrifuged,and the supernatants were purified throught he process involving ammonium sulfate precipitation,ultrafiltration,Sephadex G-75 filtration,DEAE-Sepharose Fast Flow anion exchange.The enzyme of electrophoretic homogeneity can degrade aflatoxin M_1 and the degradation rate was 28.9%.The apparent molecular weight was 58 kuas determined by SDS-PAGE.

关 键 词：黄曲霉毒素M1 分离纯化 短小芽孢杆菌 脱毒 高效液相色谱 

分 类 号：TS201.3[轻工技术与工程—食品科学]