6种食用花卉有效成分及其抗氧化活性研究  

Study on the bioactive ingredients and antioxidant activities of 6 kinds of edible flowers

作　　者：张东峰[1] 刘琪 张欣悦 张佳琦[2] 付鑫景 陆俊[2,3] ZHANG Dong-feng;LIU Qi;ZHANG Xin-yue;ZHANG Jia-qi;FU Xin-jing;LU Jun(Department of Food and Biological Engineering,Guangdong Industry Technical College,Guangzhou,Guangdong 510300,China;College of Food Science and Technology,Central South University of Forestry and Technology,Changsha ＆ Hunan 410004,China;Hunan Key Laboratory of Processed Food for Special Medical Purpose,Changsha,Hunan 410004,China)

机构地区：[1]广东轻工职业技术学院食品与生物工程系,广东广州510300 [2]中南林业科技大学食品科学与工程学院,湖南长沙410004 [3]特医食品加工湖南省重点实验室,湖南长沙410004

出　　处：《食品与机械》2018年第9期167-171,184,共6页Food and Machinery

基　　金：中南林业科技大学大学生研究性学习与创新性实验计划项目(编号:107-0167);湖南省科技创新平台与人才计划项目(编号:2017TP1021)

摘　　要：利用超声波法对6种常见食用花卉的活性成分进行提取,并对其活性成分和抗氧化活性进行测定。结果表明,6种食用花卉中多酚、黄酮、花色苷和类胡萝卜素活性成分含量范围分别为6.20～63.17 mg GAE/g·DW,12.52～180.47mg CE/g·DW,4.17～40.91 mg CyA/g·DW,0.33～0.68mg/g·DW。DPPH自由基清除能力为45.13～1 092.49μmol Trolox(TE)/g·DW,亚铁离子还原能力(FRAP)为100.20～2 621.25μmol TE/g·DW,以及ABTS自由基清除能力为100.12～1 708.03μmol TE/g·DW。3种抗氧化活性中,DPPH自由基清除力最强的为橙色万寿菊,亚铁离子还原能力和ABTS自由基清除能力最强的均为黄色万寿菊。高效液相色谱测定6种花卉样品中主要的多酚和黄酮类化合物为阿魏酸、没食子酸和槲皮素,其中以橙色万寿菊和黄色万寿菊的活性成分含量最丰富。In this study,the bioactive compounds of 6 common edible flowers were extracted by ultrasonic extraction method and their antioxidant activities were assessed.The content of flower active ingredients such as polyphenols,flavonoids,anthocyanins and carotenoids were determined by ultraviolet spectrophotometry and HPLC.The content of these active compounds range from 6.20～63.17 mg GAE/g·DW,12.52～180.47 CE/g· DW,4.17～40.91 mg CyA/g·DW and 0.33～0.68 mg/g ·DW,respectively.The antioxidant activities were assessed by DPPH,FRAP and ABTS assays,range from 45.13～1 092.49,100.20～2 621.25 and 100.12～1 708.03μmol TE/g DW respectively.The highest DPPH radical scavenging activity was found in orange Tagetes erecta L.,and the highest ABTS and FRAP radical scavenging activity were found in yellowTagetes erecta L.,.HPLC determination results showed that the main polyphenols and flavonoids of these edible flowers were ferulic acid,gallic acid and quercetin.Among these 6 kinds of edible flower,oange Tagetes erecta L.and yellowTagetes erecta L.contain the most abundant active ingredient.

关 键 词：食用花卉 抗氧化活性 活性成分 万寿菊 桂花 

分 类 号：TS201.2[轻工技术与工程—食品科学]