壳聚糖固定化β-半乳糖苷酶的研究  

The immobilization of β - galactosidase on chitosan

作　　者：敖海英[1] 张洋[1] 李红[1] 赵征[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《中国乳品工业》2002年第5期27-29,33,共4页China Dairy Industry

摘　　要：以壳聚糖-戊二醛吸附交联法对β-半乳糖苷酶进行固定化方法的研究,获得了最优固定化条件:采用先固定化后交联的顺序,4℃固定化6h,pH值为6.0,质量分数为1％戊二醛室温交联30min,最适加酶量为0.4 mg/g干壳聚糖珠,结果酶活力回收率达到26.43％。β- galactosidase from aspergillus oryzae was adsorbed on chitosan and crossinked with glutaraldehyde. The optimal condiitons in the experiment were immobilization followed with crosslinking, under 4℃ temperature immobilization for 6 h, pH = 6. 0, 1 % glutaraldehyde under room temperature for 30 min. The amount of β - galactosidase immobilzed on chitosan was 0. 4 mg/g dry chitosan beads and the activity yield reached 26. 43% .

关 键 词：Β-半乳糖苷酶 壳聚糖 固定化 功能性乳制品 乳清粉 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程] Q814[轻工技术与工程—食品科学与工程]