蓝圆鲹蛋白酶解物的螯合矿物离子活性研究  

Mineral Ion Chelating Activity of Enzymatic Protein Hydrolysates from Decapterus maruadsi Muscle

作　　者：杨伊然[1,2] 胡晓[1] 杨贤庆[1] 李来好[1] 陈胜军[1] 吴燕燕[1] 林婉玲[1] 黄卉[1] 马海霞[1] YANG Yiran;HU Xiao;YANG Xianqing;LI Laihao;CHEN Shengjun;WU Yanyan;LIN Wanling;HUANG Hui;MA Haixia(Key Laboratory of Aquatic Producut Processing, Ministry of Agriculture, National R&D Center for Aquatic Product Processing,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciencces, Guangzhou 510300, China;College of Food Science and Engineering, Dalian Ocean University, Dalian 116023)

机构地区：[1]中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,国家水产品加工技术研发中心,广东广州510300 [2]大连海洋大学食品科学与工程学院,辽宁大连116023

出　　处：《食品科学》2017年第3期88-93,共6页Food Science

基　　金：国家海洋公益性项目(201305018);国家自然科学基金青年科学基金项目(31301454);农业部财政重大专项(NFZX2013);广东省海洋渔业科技与产业发展专项(A201401C02;Z2015008);“十二五”国家科技支撑计划项目(2015BAD17B03-02)

摘　　要：以蓝圆鲹为原料,采用胰蛋白酶、木瓜蛋白酶及碱性蛋白酶对其进行水解,研究酶解产物与钙、亚铁、锌离子的螯合活性。结果表明:蓝圆鲹经胰蛋白酶水解,水解度为23.14%时其产物具有较高螯合铁、锌离子的活性,其螯合率分别高达96.63%和94.28%;蓝圆鲹经木瓜蛋白酶水解,水解度为22.00%时,其产物具有较高螯合钙离子的活性,其螯合率高达96.78%;同时研究表明上述两种酶解物还具有较好的抗氧化活性,其1,1-二苯基-2-三硝基苯肼半抑制浓度值分别为3.74 mg/m L和3.64 mg/m L。此外,氨基酸分析表明蓝圆鲹高螯合矿物离子活性的酶解物中天冬氨酸、谷氨酸、赖氨酸含量较高。In this research, we studied the chelating activity of Decapterus maruadsi muscle hydrolyzed by three different proteases, trypsin, papain and alcalase, towards Ca2+, Fe2+ and Zn2+. The results showed that the hydrolysates produced by trypsin (DH = 23.14%) had the highest chelating activity towards Fe2+ and Zn2+ with chelating rates of 96.63% and 94.28%,respectively. The hydrolysates by papain (DH = 22.0%) had the highest Ca2+ chelating activity with a chelating rate of 96.78%. It was also shown that both enzymatic hydrolysates had good antioxidant activity. The half maximal inhibitory concentration (IC50) values of the trypsin and papain hydrolysates for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were 3.74 and 3.64 mg/mL, respectively. In addition, the amino acid sequence analysis showed that the hydrolysates with higher contents of Glu, Asp and Lys had higher mineral ion chelating activity.

关 键 词：蓝圆鲹 酶解物 螯合活性 矿物离子 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学]