不同液态发酵基质对红曲霉产红曲色素及桔霉素的影响  

Effect of different liquid fermentation substrate on production of pigments and citrinin in Monascus

作　　者：吴宏[1,2] 代文婷[1,2] 连喜军[3] 郭安民[1,2] 吴洪斌[1,2] WU Hong;DAI Wenting;LIAN Xijun;GUO Anmin;WU Hongbin(Instiute of Agro-Products Processing Science and Technology,Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000,China;Key Laboratory of Agro-Products Processing Science and Technology,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China;Tianjin University of Commerce,The Tianjin Key Laboratory of Food Biotechnology,Tianjin 300134,China)

机构地区：[1]新疆农垦科学院农产品加工研究所,新疆石河子832000 [2]新疆农垦科学院农产品加工重点实验室,新疆石河子832000 [3]天津商业大学天津市食品生物技术重点实验室,天津300134

出　　处：《中国酿造》2018年第11期91-94,共4页China Brewing

基　　金：国家自然科学基金资助项目(31260396)

摘　　要：在前期分离纯化出的10株红曲霉基础上,以18种液体培养基对10株红曲霉进行发酵培养,通过分光光度计和质谱分析仪测定各发酵液中红曲色素和桔霉素含量,分析不同地区红曲霉的红曲色素和桔霉素的代谢特性,筛选出高产红曲色素低产桔霉素的红曲霉菌株。结果表明,产红曲色素最适的液体培养基配方为葡萄糖3%、蛋白胨1%,其中新疆地区红曲霉所产红曲色素量最高,为6.81×10-2mg/m L;新疆地区红曲霉仅在18种培养基中的面筋碱性蛋白酶水解液+葡萄糖发酵液中产生了桔霉素,而红曲霉ZBX天津在所有培养基发酵液中均未产生桔霉素。Based on 10 strains of Monascus isolated and purified in the early stage,the 10 Monascus strains were fermented and cultured in 18 kinds of liquid media,and the Monascus pigments and citrinin contents in the fermented liquid were determined by spectrophotometer and mass spectrum analyzer.The metabolic properties of Monascus pigments and citrinin in the Monascus from different regions were analyzed,and a high-yield pigment but lower citrinin strain was screened.Experimental results showed that the optimal liquid medium formula was glucose 3%and peptone 1%.The contents of Monascus pigments by strains of Xinjiang region were the highest,with the concentration of 6.81×10-2 mg/ml.Citrinin was only detected in the medium of hydrolytic solution of gluten alkaline protease and glucose fermentation liquid,and Tianjin Monascus ZBX did not produce citrinin in all fermentation liquid.

关 键 词：红曲霉 红曲色素 桔霉素 

分 类 号：TQ925.7[轻工技术与工程—发酵工程]