黑芸豆肽饮料的研制  

Preparation of Black Kidney Bean Peptide Beverage

作　　者：杨旭[1] 李菁[2] 孙先保 杨柳[3] 徐尚英 何述栋[3] YANG Xu;LI Jing;SUN Xianbao;YANG Liu;XU Shangying;HE Shudong(Anhui Baidi Dairy Co.,Ltd. of New Hope Group,Hefei,Anhui 231600,China;Department of Biological and Environmental Engineering,Hefei University,Hefei,Aihui 230009,China;School of Food Science and Biology Engineering,Hefei University of Technology,Hefei,Anhui 230009,China;Anhui Sea God Yellow Wine Group Co.,Ltd.,Hefei,Anhui 231561,China)

机构地区：[1]安徽新希望白帝乳业有限公司,安徽合肥231600 [2]合肥学院生物与环境工程系,安徽合肥230009 [3]合肥工业大学食品与生物工程学院,安徽合肥230009 [4]安徽海神黄酒集团有限公司,安徽合肥231561

出　　处：《农产品加工》2019年第11期12-16,共5页Farm Products Processing

基　　金：安徽省自然科学基金项目“基于‘双提高’策略的PEG蛋白质修饰对芸豆凝集素致敏性调制的机制研究”(1708085MC70);安徽省科技攻关计划项目“大米酿造料酒产业化关键技术及料酒生产车间数字化研究”(16030701084)

摘　　要：通过风味蛋白酶对黑芸豆蛋白进行酶解制备黑芸豆肽,并以此为主要原料研制黑芸豆肽饮料。通过响应面分析优化了黑芸豆蛋白酶酶解的工艺条件,基于正交试验优化了黑芸豆肽饮料的配方。结果表明,当酶添加量4%,酶解温度55℃,酶解时间180min时,黑芸豆蛋白的酶解度最高,黑芸豆肽的抗氧化性最强;当黑芸豆肽添加量60%,白砂糖添加量15%,柠檬酸添加量0.03%时,黑芸豆肽饮料的口感最佳。The black kidney beans were investigated in the experiment.The black kidney bean peptide was prepared by hydrolysis of the flavor protease,and the black kidney bean peptide beverage was prepared by using the hydrolysis product as the main raw material.Based on the previous single factor experiments,the process conditions of black kidney bean hydrolysis were optimized by response surface analysis,and the formulation of black kidney bean peptide beverage was optimized by orthogonal experiment.The results showed that the best hydrolysis conditons were the enzyme dosage 4%,the hydrolysis temperature 55℃,the hydrolysis time 180 min,the hydrolysis degree of black kidney bean protein was the highest,and the antioxidant activity of black kidney bean peptide was the strongest;meanwhile the addition amount of black kidney bean peptide was 60%,the addition amount of sugar was 15%and the amount of citric acid added was 0.03%,the taste of the black kidney peptide drink was the best.

关 键 词：黑芸豆 蛋白肽 饮料 酶解度 抗氧化性 感官评价 

分 类 号：TS273[轻工技术与工程—农产品加工及贮藏工程] TS278[轻工技术与工程—食品科学与工程]