SDE和HS-SPME结合GC-MS分析霍山黄大茶香气成分的比较  

Comparison of Simultaneous Distillation and Extraction and Headspace-Solid Phase Microextraction for Analysis of Aroma Components of Huoshan Large-Leaf Yellow Tea by Gas Chromatography-Mass Spectrometry

作　　者：朱晓凤[1] 刘政权[1] 宛晓春[1] 陶勐 肖志鹏 程淑华 ZHU Xiaofeng;LIU Zhengquan;WAN Xiaochun;TAO Meng;XIAO Zhipeng;CHENG Shuhua(State Key Laboratory of Tea Plant Biology and Utilization,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学茶树生物学与资源利用国家重点实验室

出　　处：《食品科学》2020年第4期214-221,共8页Food Science

基　　金：安徽省科技重大专项(18030701141);现代农业(茶叶)产业技术体系建设专项(CARS-23);2016—2020年安徽省茶产业体系茶叶深加工岗位专家项目(11250009)

摘　　要：分别采用同时蒸馏萃取法(simultaneous distillation and extraction,SDE)和顶空固相微萃取法(headspacesolid phase microextraction,HS-SPME)提取霍山黄大茶中的香气成分,通过气相色谱-质谱定性定量分析,对2种方法所得的香气成分进行比较。结果表明,2种方法提取的香气成分和相对含量有很大差异,SDE法检测出的挥发性成分有74种,HS-SPME法检测到的挥发性成分有76种,2种方法共同检出的组分有44种。糠醛、5-甲基呋喃醛、3-乙基-2,5-二甲基吡嗪、水杨酸甲酯等是构成霍山黄大茶香气的重要物质,2种方法测出的相对含量都在2%以上。2种方法特有香气化合物分别有30、32种,苯乙烯、苯乙醛、顺式芳樟醇氧化物(呋喃)、亚麻酸等仅在SDE法中检出,HS-SPME法中特有苯甲醛、十四烷、香叶基丙酮等香气组分。Comparative analysis of the aroma components of Huoshan large-leaf yellow tea was performed by simultaneous distillation and extraction(SDE)and headspace-solid phase microextraction(HS-SPME)combined with gas chromatography-mass spectrometry(GC-MS).The results showed that the composition and contents of volatile compounds detected by the two methods were significantly different.A total of 74 compounds were obtained by SDE while 76 compounds by HS-SPME.In total,44 components were detected by both methods.Furfural,5-methyIfurfural,3-ethyl-2,5-dimethylpyrazine and methyl salicylate were important substances that contributed to the unique aroma of large-leaf yellow tea and their relative contents were all over 2%obtained by both methods.Furthermore,30(styrene,benzeneacetaldehyde,cis-linaloloxide(furanoid),and linolenicacid)and 32(benzaldehyde,tetradecane and geranylacetone)aroma components were exclusively detected by SDE and HS-SPME,respectively.

关 键 词：霍山黄大茶 香气 同时蒸馏萃取法 顶空固相微萃取 气相色谱-质谱 

分 类 号：S571.1[农业科学—茶叶生产加工] TS272[农业科学—作物学]