维生素E纳米乳液冻干粉末的制备及结构特性研究  

Studies on Preparation and Structural Properties of Vitamin E Nanoemulsion Freeze-dried Powder

作　　者：张潇元[1] 潘悦 朱鸿维 王中江[1] 江连洲[1] 谢凤英[1] 赵玉莹 张晓南[1,2] hang Xiaoyuan;Pan Yue;Zhu Hongwei;Wang Zhongjiang;Jiang Lianzhou;Xie Fengying;Zhao Yuying;Zhang Xiaonan(College of Food Science,Northeast Agricultural University,Harbin 150030;Heilongjiang Xueqin Technology Co.,Ltd,Harbin 150030)

机构地区：[1]东北农业大学食品学院,哈尔滨150030 [2]黑龙江省学勤科技有限公司,哈尔滨150030

出　　处：《中国食品学报》2020年第8期118-129,共12页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自科学基金青年科学基金项目(331701568);黑龙江省自然科学基金面上项目(C2018025);“十三五”国家重点研发计划项目(2016YFD 0400402-02)。

摘　　要：联合真空冷冻干燥技术与高压均质技术制备一种水包油型(O/W)真空冷冻干燥维生素E纳米乳液粉末复原乳液,通过单因素试验及响应面分析法优化该复原乳液的最佳工艺条件,并通过扫描电子镜、流变仪、红外光谱等手段对维生素E纳米乳液、冷冻干燥粉末以及复原乳液的物理化学性质进行研究。结果表明:维生素E纳米乳液粉末复原乳液最佳工艺条件为:大豆分离蛋白质量浓度15 mg/mL,葵花籽油质量浓度45 mg/mL,均质压力100 MPa,均质4次。所得维生素E纳米乳液粉末复原乳液平均粒径232.66 nm,包埋率90.82%。冻干粉末复原乳液稳定性良好,扫描电镜结果显示冻干粉末结构致密均匀、表面光滑;流变仪扫描结果显示冻干粉末复原乳液呈典型的剪切变稀流变行为,具有牛顿流体特征;红外光谱试验结果显示冻干粉末的蛋白α-螺旋、β-转角结构增加,β-折叠、无规则卷曲结构降低。真空冷冻干燥的维生素E纳米乳液粉末复原乳液,提高了维生素E吸收率且乳液体系稳定性良好。Vacuum freeze-drying vitamin E nanoemulsion powder reconstituted emulsion(O/W)was prepared by combining vacuum freeze-drying and high pressure homogenization technology,with the optimum technological conditions optimized by single factor experiment and response surface methodology.Further,the physical and chemical properties of it were studied by scanning electron microscopy,rheometer and infrared spectroscopy,etc.And the research results showed that the optimum technological conditions were:The mass concentration of SPI and sunflower seeds oil is respectively 15 mg/mL and 45 mg/mL,while the homogenization pressure and times is 100 MPa and four times.The average particle size was 232.66 nm and the embedding rate was 90.82%.And the vacuum freeze-drying powder reconstituted emulsion has good stability,and the scanning electron microscopy results showed it had a compact structure and a smooth surface.The rheometer scanning results showed that the freeze-drying powder reconstituted emulsion showed a typical rheological behavior of shear thinning,with Newtonian fluid characteristics.The results of infrared spectroscopy showed that theα-helix andβ-turn structure of the freeze-dried powder increased,while theβ-sheet and random coil structure decreased.Inconclusion,the vacuum freeze-drying vitamin E nanoemulsion powder reconstituted emulsion improves the vitamin E absorption ratio and the stability of the emulsion system.

关 键 词：维生素E 大豆分离蛋白 真空冷冻干燥 高压均质 

分 类 号：TS205[轻工技术与工程—食品科学]