降解黄曲霉毒素B1土曲霉发酵工艺优化的研究  

Study on Optimization of Fermentation Process of Aflatoxin B1 Degradation by Aspergillusterreus

作　　者：宁梦鸽 谢岩黎[1] 孙淑敏[1] 马卫宾 李倩 卫敏[1] 何保山[1] 任文洁 NING Meng-ge;XIE Yan-li;SUN Shu-min;MA Wei-bin;LI Qian;WEI-Min;HE Bao-shan;REN Wen-jie(College of Cereals,Oils and Foods,Henan Key Laboratory of Cereals,Oils and Foods Safety Inspection and Control,Henan University of Technology,Zhengzhou,Henan 450001,China)

机构地区：[1]河南工业大学粮油食品学院,河南省粮油食品安全检测与控制重点实验室,河南郑州450001

出　　处：《粮油食品科技》2020年第5期18-23,共6页Science and Technology of Cereals,Oils and Foods

基　　金：河南省高校科技创新团队计划资助(20IRTSTHN023);河南省重点研发与推广专项(192102310254)。

摘　　要：对黄曲霉毒素B1(Aflatoxin B1,AFB1)降解菌土曲霉(Aspergillusterreus)HNGD-TM15降解AFB1发酵工艺进行优化。在单因素实验基础上,运用Box-Behnken设计原理,对降解AFB1影响显著的HNGD-TM15发酵工艺参数碳源(葡萄糖)、pH和温度设计响应面实验,结果表明:模型(P=0.0028)在1%水平上具有极显著性,失拟项不显著(P=0.0635>0.05),其R2=0.9299,R2 Adj=0.8399,说明该模型拟合程度良好,可以模拟92.99%的AFB1降解率变化,其中pH对AFB1降解率的影响最大。确定HNGD-TM15发酵主要参数:葡萄糖0.7%,发酵液起始pH为3.0,发酵温度为34℃,接种量为0.006%的菌悬液,装液量为100 mL/250 mL,发酵时间为72 h。优化后AFB1降解率从接种量为5%的98.30%提高到接种量为0.006%的99.94%。The fermentation process of Aflatoxin B1(Aflatoxin B1,AFB1)degrading bacteria Aspergillusterreus HNGD-TM15 to degrade AFB1 was optimized.Based on the single-factor experiment,the Box-Behnken design principle was used to design the response surface experiment for the HNGD-TM15 fermentation process parameters,including carbon source(glucose),pH and temperature,which can significantly affect the degradation of AFB1.The results showed that the model(P=0.0028)was extremely significant at 1%level,and the lack-of-fit term is not significant(P=0.0635>0.05)with R2=0.9299 and R2 Adj=0.8399,indicating that the model fits well,and it can simulate 92.99%of AFB1 degradation rate changes,in which pH has the greatest effect on the degradation rate of AFB1.The fermentation parameters of HNGD-TM15 were determined as follows:glucose was 0.7%,the initial pH of the fermentation broth was 3.0,the fermentation temperature was 34℃,the inoculation volume was 0.006%of the bacterial suspension,and the filling volume was 100 mL/250 mL,fermentation time was 72 h.After optimization,the degradation rate of AFB1 was increased from 98.30%with an inoculation volume of 5%to 99.94%with an inoculation volume of 0.006%.

关 键 词：黄曲霉毒素B1 发酵工艺 优化 响应面设计 降解 

分 类 号：TS209[轻工技术与工程—食品科学]