小麦蛋白水解物对肠道上皮细胞抵御氧化应激损伤的作用  

Effect of Wheat Protein Hydrolysates on the Resistance of Intestinal Epithelial Cell to Resist Oxidative Stress

作　　者：岳颖 严斌[1,2] 刘丽娅 庞淑婕[1] 周闲容 佟立涛[1] 王丽丽[1] 周素梅[1] Yue Ying;Yan Bin;Liu Liya;Pang Shujie;Zhou Xianrong;Tong Litao;Wang Lili;Zhou Sumei(Institute of Food Science and Technology CAAS,Key Laboratory of Agro-products Processing,Ministry of Agriculture,Beijing 100193;Hunan Prospecting Designing&Research General Institute for Agriculture Forestry&Industry,Changsha 410007)

机构地区：[1]中国农业科学院农产品加工研究所,农业部农产品加工重点实验室,北京100193 [2]湖南省农林工业勘察设计研究总院,长沙410007

出　　处：《中国食品学报》2020年第10期68-75,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：农业部948项目(2016-X31);中国农业科学院科技创新工程项目(CAAS-ASTIP-201X-IAPPST)。

摘　　要：采用双酶和三酶对小麦蛋白复合水解,获得富含谷氨酰胺(Gln)的双酶酶解产物(PWGH)和三酶酶解产物(AWGH)。比较小麦蛋白及其2种酶解产物经体外模拟消化前、后,抵御肠上皮细胞(Caco-2)氧化应激损伤能力的变化。结果表明:2种水解产物均能在一定程度上缓解细胞因氧化应激损伤导致的存活率降低现象,减少细胞氧化损伤的程度。二者经体外模拟消化降解后仍有此作用,而小麦蛋白的体外模拟消化产物对损伤细胞存活率无显著影响。2种水解产物均能在一定程度上提高氧化应激损伤细胞的抗氧化能力,提高细胞内还原型谷胱甘肽(GSH)水平,减少损伤细胞的脂质氧化,然而,经体外模拟消化降解后,PWGH仍有上述作用,而AWGH对损伤细胞的抗氧化能力和GSH水平无显著影响。研究表明:体外模拟消化对AWGH的活性肽段有破坏,而PWGH活性肽段经消化后仍有保留。值得注意的是,小麦蛋白经体外模拟消化,其产物(WGH)虽能减少损伤细胞的脂质氧化,但对损伤细胞的抗氧化能力和GSH水平均无显著影响。Wheat protein hydrolysis rich in glutamine were obtained by two(PWGH)or three-enzymes(AWGH)combined hydrolyzed methods.Then their abilities in resisting the oxidative stress of intestinal cells(Caco-2)were studied after the digestion in vitro.The results showed that both of PWGH and AWGH could alleviate the decrease of cell survival rate,and reduce the degree of cells damage.Moreover,the hydrolysis still maintained their activities after digestion,but the digestion of wheat protein showed no effect on the cell survival rate.PWGH and AWGH both improved the cellular antioxidant capacity,with an increase of intracellular GSH and decrease of lipid oxidation of damaged cells.The PWGH still had the function after digestion,but AWGH had no effect on the cellular antioxidant capacity and the level of GSH on damaged cells after digestion.It indicated that some active fragment in AWGH might be damaged after digestion,but not for the digestion of PWGH.Besides,it is worthy to note although the digestion of wheat protein could reduce the lipid oxidation of damaged cells,it had no effect on the cellular antioxidant capacity and GSH level.

关 键 词：小麦蛋白 酶解 体外消化 氧化应激 抗氧化 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]