量子点标记荧光免疫试纸条可视化检测肉品中志贺氏菌研究  

Study on Visual Detection of Shigella by Quantum Dot Labeling with Fluorescence Immunoassay Strip in Meat

作　　者：朱芳茜 何扩[1] 张秀媛[1] 魏东[1] 王硕[2] 李育峰[1] 陈一[1] 赵瑞平[1] ZHU Fang-xi;HE Kuo;ZHANG Xiu-yuan;WEI Dong;WANG Shuo;LI Yu-feng;CHEN Yi;ZHAO Rui-ping(Hebei Key Laboratory of Quality&Safety Analysis-Testing for Agro-Products and Food,Hebei North University,Zhangjiakou 075000,Hebei,China;Nankai University,Tianjin 300000,China)

机构地区：[1]河北省农产品食品质量安全与检测重点实验室,河北北方学院,河北张家口075000 [2]南开大学,天津300000

出　　处：《食品研究与开发》2021年第3期136-140,共5页Food Research and Development

基　　金：河北省引进国外智力项目;河北省农业科技成果转化资金项目(19822801D、20822906D);石家庄市科学技术研究与发展计划项目(191170034A、191170082A);石家庄市引进国外智力项目;河北省自然科学基金项目(C2020405003);石家庄市科技孵化计划项目(191540119A)。

摘　　要：志贺氏菌是一类具有强传染性和危害严重的革兰阴性食源性致病菌。该文利用自制的CdTe量子点偶联志贺氏菌单克隆抗体,将志贺氏菌单克隆抗体和羊抗鼠二抗分别喷涂硝酸纤维素膜,并分别作为试纸条检测线和质控线,研制一种可视化检测志贺氏菌的量子点免疫荧光试纸条。该试纸条对肉类实际样品的检测限为1×104 CFU/mL,单个样品检测时间只需15 min,对常见的5种食源性致病菌均无交叉反应性。该方法构建的基于量子点免疫荧光试纸条满足快速、可视化、高灵敏检测志贺氏菌需求,可用于肉类食品的快速检测。The Shigella is a kind of gram-negative food-borne pathogenic bacteria with strong infectivity and serious harm.In this study,self made CdTe quantum dots were electrostatic coupled with mouse anti-Salmonella monoclonal antibody.Anti-Salmonella monoclonal antibody and sheep anti-mouse antibody were marked on the membrane as test line and control line,separately.A quantum dot immunofluorescence strip was developed for the visual detection of Shigella. the fluorescent immunostrip exhibited a good sensitivity with a limit of detection of 1×104 CFU/mL. The detection time of the proposed fluorescent immunostrip for each sample was only 15 min. The fluorescent immunostrip was used to detect five common foodborne pathogens, and the results showed that there were no cross-reactions. The prepared fluorescent immunostrip could provide a visual and highly accurate method for the detection of Shigella in meat.

关 键 词：志贺氏菌 量子点 试纸条 肉类食品 检测 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]