半胱氨酸处理对采后金针菇品质和抗氧化活性的影响  

Effects of Cysteine Treatment on the Quality and Antioxidant Activity in Postharvest Flammulina velutipes

作　　者：侯权 窦立耿 钱霄晨 张之涵 姜天甲[1] HOU Quan;DOU Ligeng;QIAN Xiaochen;ZHANG Zhihan;JIANG Tianjia(College of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China;Lianyungang Nanyuntai Forest Farm Management,Lianyungang 222062,China)

机构地区：[1]浙江工商大学食品与生物工程学院,浙江杭州310018 [2]连云港市南云台林场管理处,江苏连云港222062

出　　处：《食品工业科技》2021年第16期303-310,共8页Science and Technology of Food Industry

基　　金：浙江省自然科学基金(LY21C200009)。

摘　　要：为了研究半胱氨酸处理对金针菇低温贮藏品质和抗氧化活性变化的影响,将新鲜金针菇分别采用15 mmol/L半胱氨酸和清水浸泡处理20 min后于2~3℃贮藏12 d,并定期检测相关理化指标。结果表明,15 mmol/L半胱氨酸处理能够抑制新鲜金针菇的呼吸强度和褐变度;延缓可溶性固形物和可溶性蛋白含量下降,并保持较低相对电导率;抑制了多酚氧化酶(polyphenol oxidase,PPO)活性和过氧化物酶(peroxidase,POD)活性,并保持较高的总酚和抗坏血酸含量,贮藏结束时,半胱氨酸处理组的PPO和POD活性比对照组分别降低了13.78%和26.49%,总酚和抗坏血酸含量分别为对照组的1.12和1.37倍;处理组提高了超氧化物歧化酶(superoxide dismutase,SOD)活性、谷胱甘肽还原酶活性以及1,1-二苯基-2-三硝基苯肼自由基(1,1-diphenyl-2-picrylhydrazyl,DPPH)清除率,对失重率无显著影响;相关性分析发现,处理组的呼吸强度与SOD活性、DPPH自由基清除率相关系数分别为−0.951和−0.903,呈显著(P<0.05)负相关。由此得出,低温贮藏期间,半胱氨酸处理能够降低金针菇内部物质的代谢速率,维持较高抗氧化能力和感官品质。In order to investigate the effects of cysteine(Cys)treatment in preserving quality and antioxidant activities of Flammulina velutipes during low temperature storage,fresh Flammulina velutipes were pretreated with 15 mmol/L cysteine and clear water at 25℃for 20 min before storage for 12 d at 2~3℃,and The physical and chemical indexes were tested regularly.The results showed that 15 mmol/L cysteine treatment could restrain the respiration intensity and delay browning degree of fresh mushroom.At the same time,cysteine treatment could reduce the rate of decline of soluble solids and soluble protein.And the relative conductivity could be kept at low state in cysteine treated mushroom.And cysteine treatment significantly controlled the activity of the polyphenol oxidase(PPO)and could down-regulate the activity of peroxidase(POD).In addition,cysteine treatment had reserved thephenolic compound and ascorbic acid content.At 12 days of shelf-life,the activities of PPO and POD in cysteine treatment group decreased 13.78%and 26.49%compared with the control group,respectively,and the contents of total phenol and ascorbic acid by cysteine treated were 1.12 and 1.37 times of those in control group.Moreover,it could maintain higher levels of superoxide dismutase(SOD)and glutathione reductase(GR)activity along with superior 1,1-diphenyl-2-picrylhydrazyl(DPPH)scavenging capacity.However,it had no significant difference on the weight loss rate between the two treatment groups.The correlation analysis showed that the correlation coefficients between respiratory intensity and SOD activity and DPPH scavenging rate in the treatment group were−0.951 and−0.903,respectively,and showed a significant negative correlation.In conclusion,cysteine treatment could reduce the metabolic rate of internal substances of Flammulina velutipes,maintain higher antioxidant capacity and sensory quality during preserved period.

关 键 词：金针菇 半胱氨酸 抗氧化活性 保鲜 褐变 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]