低聚半乳糖对植物乳杆菌发酵乳特性及抗蜡样芽孢杆菌活性的改善  

The Improvement of Lactobacillus plantarum Fermented Milk in Characterization and Anti-Bacillus cereus Activity by Galactooligosacchari

作　　者：刘乐 邹开翔 邵开生 于晖[2] 陶雪莹[1] 魏华[1,3] 张志鸿[1,3] LIU Le;ZOU Kaixiang;SHAO Kaisheng;YU Hui;TAO Xueying;WEI Hua;ZHANG Zhihong(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;Jiangxi Provincial Center for Disease Control and Prevention,Nanchang 330029,China;Jiangxi-OAI Joint Research Institute,Nanchang Univerisity,Nanchang 330047,China)

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]江西省疾病预防控制中心,江西南昌330029 [3]南昌大学中德联合研究院,江西南昌330047

出　　处：《食品工业科技》2022年第15期139-147,共9页Science and Technology of Food Industry

基　　金：国家自然基金青年科学基金项目(32101915);江西省自然科学基金项目(20212BAB215033)。

摘　　要：为探究低聚半乳糖对植物乳杆菌发酵乳特性及抗菌活性的影响,本文采用单因素法考察影响发酵乳特性的主要因素,并以响应面法优化发酵乳最佳发酵条件;以产肠毒素蜡样芽孢杆菌HN001为指示菌,探究低聚半乳糖的添加对植物乳杆菌ZDY2013发酵乳抑菌活性的改善作用。结果表明:植物乳杆菌能有效利用低聚半乳糖进行体外代谢,并抑制蜡样芽孢杆菌生长;牛奶中添加适量低聚半乳糖能够增加植物乳杆菌发酵乳中的活菌数、降低发酵乳的pH,并提高其持水力;响应面分析发现低聚半乳糖发酵乳的最佳制备条件为:2.0%的植物乳杆菌接种量、1.0%的低聚半乳糖添加量、发酵时间为24 h及发酵温度为42℃;添加低聚半乳糖的发酵乳能有效控制产肠毒素蜡样芽孢杆菌浓度在106 CFU/mL以下。该研究结果为低聚半乳糖及植物乳杆菌ZDY2013在发酵乳中的应用奠定了理论基础。In order to test the effect of galactooligosaccharide(GOS)on the characterization and antibacterial activity of milk fermented by Lactobacillus plantarum,the main factors affecting the fermented milk were investigated and the most suitable fermentation conditions for improving the characterization of fermented milk were obtained with response surface analysis.The enterotoxigenic Bacillus cereus HN001 was used as an indicator to study the effect of GOS addition on the antibacterial activity of L.plantarum ZDY2013 fermented milk.The results showed that L.plantarum could use GOS for metabolism in vitro and inhibit the growth of B.cereus effectively.The proper addition of GOS in milk could increase the number of viable bacteria,reduce the pH and enhance the water holding capacity of L.plantarum fermented milk.The result from response surface analysis was found that the best fermentation conditions for fermented milk were 2.0%inoculums of L.plantarum,1.0%GOS,fermented at 42℃for 24 h.Additionally,GOS addition in fermented milk could effectively control the concentration of enterotoxigenic B.cereus HN001 to below 106 CFU/mL.The results of this study provide the foundation for the application of GOS and L.plantarum ZDY2013 in fermented milk.

关 键 词：低聚半乳糖 植物乳杆菌 响应面分析 发酵乳 抑菌 蜡样芽孢杆菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]