产β-葡萄糖苷酶乳酸菌益生特性研究  

Probiotic properties ofβ-glucosidase producing lactic acid bacteria

作　　者：冯程程 蔡子哲[1,2,3] 陈琼 欧仕益 谢小冬[1,2,3] 汪勇 MARTIN J T REANEY[2,3,5] 张宁 FENG Chengcheng;CAI Zizhe;CHEN Qiong;OU Shiyi;XIE Xiaodong;WANG Yong;MARTIN J T REANEY;ZHANG Ning(Department of Food Science and Engineering,Jinan University,Guangzhou 510632,China;Guangdong International Joint Research Center for Oilseeds Biorefinery,Nutrition and Safety,Guangzhou 510632,China;Jinan University-Saskatchewan Oilseed Joint Laboratory,Guangzhou 510632,China;Guangzhou Institute of Microbiology Co.,Ltd,Guangzhou 510663,China;College of Agriculture and Bioresources,University of Saskatchewan,Saskatoon S7N5A8,Canada)

机构地区：[1]暨南大学食品科学与工程系,广东广州510632 [2]广东省油料生物炼制与营养安全国际联合研究中心,广东广州510632 [3]暨南大学-萨斯喀彻温大学“油料生物炼制与营养”联合实验室,广东广州510632 [4]广州市微生物研究所有限公司,广东广州510663 [5]加拿大萨斯喀彻温大学农业与生物资源学院,加拿大萨斯卡通S7N5A8

出　　处：《食品与发酵工业》2022年第15期85-90,共6页Food and Fermentation Industries

基　　金：国家重点研发计划政府间国际创新合作重点专项(2018YFE0108400);广州市科技计划项目民生科技攻关计划项目(201903010049)。

摘　　要：研究了3株乳酸菌在不同碳源诱导下产β-葡萄糖苷酶的能力,并评价了其益生特性。研究发现,3株乳酸菌产生的β-葡萄糖苷酶同时具有水解烷基糖苷和芳香基糖苷的能力,纤维二糖诱导能显著提高β-葡萄糖苷酶活力。坚强肠球菌(Enterococcus durans)GW18275在纤维二糖为碳源时有最大β-葡萄糖苷酶活力(16.02 U/mL)。模拟胃肠液耐受试验结果表明,3株乳酸菌活菌数保持在10^(6) CFU/mL以上。植物乳植杆菌(Lactiplantibacillus plantarum)C5在3 g/L胆盐培养3 h有最大存活率(83.70±6.21)%,对二甲苯和乙酸乙酯的表面疏水性最佳。植物乳植杆菌(Lactiplantibacillus plantarum)C1有最高的自聚集率(80.72±1.58)%。此外,3株菌均无溶血性,对头孢菌素类、β-酰胺类及氨基糖苷类抗生素有不同的药敏性,对四环素均敏感。3株乳酸菌具有较高的β-葡萄糖苷酶活性,作为功能性益生菌候选物具有良好的益生特性,可为日后开发高产β-葡萄糖苷酶益生制剂提供基础。The production capability ofβ-glucosidase induced by different carbon sources and probiotic characteristics of three lactic acid bacteria strains were investigated.Results showed that theβ-glucosidase produced by the three strains was capable of hydrolyzing both alkyl glucoside and aryl glucoside,and the enzyme activity was significantly increased via the induction of cellobiose(P<0.05).The maximumβ-glucosidase activity(16.02 U/mL)was observed with Enterococcus durans GW18275 when it was cultured in MRS with the carbon source of cellobiose.Simulated gastroenteric fluid tolerance test found that the number of viable bacteria of three lactic acid bacteria strains in simulated gastroenteric fluid remained above 106 CFU/mL.Lactiplantibacillus plantarum C5 possessed the highest survival rate(83.70±6.21)%cultured by 0.3%bile salt for 3 h,and existed the best surface hydrophobicity to xylene and ethyl acetate.Lactiplantibacillus plantarum C1 showed the highest auto-aggregation rate(80.72±1.58)%.Moreover,the three strains were non-hemolytic and had different drug sensitivity to cephalosporins,β-lactams and aminoglycoside antibiotics,as well as possessing sensitivity to tetracycline.Given these results,the three lactic acid bacteria strains presented highβ-glucosidase activity,and had good probiotic properties as candidates of functional probiotics,which can be used to develop new functional probiotics products.

关 键 词：Β-葡萄糖苷酶 酶活力 乳酸菌 益生特性 模拟胃肠液 

分 类 号：TS201.3[轻工技术与工程—食品科学]