富硒辣木籽蛋白降压肽的酶法制备、硒含量及稳定性研究  

Preparation,selenium content and stability of antihypertensive peptides from selenium-enriched Moringa oleifera seed protein by enzymatic hydrolysis

作　　者：陈冰冰 杨奕 李嘉颐 金昶言 程缤霈 邓泳琪 林碧敏 梁东 唐德剑 孟莉 苗建银[1] CHEN Bing-bing;YANG Yi;LI Jia-yi;JIN Chang-yan;CHENG Bin-pei;DENG Yong-qi;LIN Bi-min;LIANG Dong;TANG De-jian;MENG Li;MIAO Jian-yin(College of Food Science South China Agricultural University,Guangzhou,Guangdong 510642,China;State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources,Guangxi Normal University,Guilin,Guangxi 541004,China;Key Laboratory of Se-enriched Products Development and Quality Control,Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Laboratory of Se-enriched Food Development,Ankang,Shaanxi 725000,China;Ankang R&D Center for Se-enriched Products,Ankang,Shaanxi 725000,China)

机构地区：[1]华南农业大学食品学院广东省功能食品活性物重点实验室,广东广州510642 [2]省部共建药用资源化学与药物分子工程国家重点实验室,广西桂林541004 [3]农业农村部富硒产品开发与质量控制重点实验室/富硒食品开发国家地方联合工程实验室,陕西安康725000 [4]安康市富硒产品研发中心,陕西安康725000

出　　处：《食品与机械》2022年第8期213-221,共9页Food and Machinery

基　　金：省部共建药用资源化学与药物分子工程国家重点实验室资助课题(编号:CMEMR2020-B_07);广西创新驱动发展专项资金项目(编号:桂科AA17202010);广西自然科学基金项目(编号:2017GXNSFFA198007);农业农村部富硒产品开发与质量控制重点实验室/富硒食品开发国家地方联合工程实验室开放课题(编号:Se-2020B04)。

摘　　要：目的:开发富硒辣木籽蛋白资源。方法:以富硒辣木籽蛋白粉为原料,通过单因素和响应面优化得到富硒辣木籽蛋白降压肽的酶解工艺条件,并对最优酶解物的ACE抑制活性、硒含量和稳定性进行分析。结果:富硒辣木籽蛋白的最优酶解工艺为酶解时间3 h,酶解温度34℃,pH 8,底物浓度7%,酶底比0.3%。此条件下水解所得降压肽的ACE的半抑制浓度为1.956 mg/mL;该降压肽中硒含量为1.394 mg/kg,是原料蛋白的1.1倍;并且该降压肽(5 mg/mL)具有良好的热稳定性和酸碱稳定性,在体外经胃肠道酶系消化酶解后,依然能保持良好的ACE抑制活性。结论:富硒辣木籽蛋白降压肽具有较强的ACE抑制活性和良好的稳定性。Objective:To develop selenium-enriched(Se-enriched)Moringa oleifera seed protein resources.Methods:Using Se-enriched Moringa oleifera seed protein powder as raw material,the enzymatic hydrolysis conditions of Se-enriched Moringa oleifera seed protein antihypertensive peptides from Se-enriched Moringa oleifera seed protein were obtained by single factor and response surface optimization,and the ACE inhibitory activity,Se content and stability of the optimal enzymatic hydrolysate were analyzed.Results:The optimal enzymatic hydrolysis conditions of Se-enriched Moringa oleifera were as follows:enzymatic hydrolysis time 3 h,temperature 34℃,pH 8,concentration of substrate 7%,enzyme to substrate ratio 0.3%.Under these conditions,the semi-inhibitory concentration of ACE of the peptides was 1.956 mg/mL.The Se content of the antihypertensive peptide was 1.394 mg/kg,which was 1.1 times that of the raw protein.Moreover,the antihypertensive peptides(5 mg/mL)had good thermal stability and acid-base stability,and could still maintain good ACE inhibitory activity after digestion by gastrointestinal enzyme system in vitro.Conclusion:The Se-enriched Moringa oleifera seed protein antihypertensive peptides obtained by response surface optimization showed strong ACE inhibitory activity and good stability.

关 键 词：富硒辣木籽 酶解 降压肽 ACE抑制活性 硒含量 稳定性 

分 类 号：TS201.2[轻工技术与工程—食品科学]