青梅酚类物质在体外模拟消化过程中生物接受率和抗氧化活性的变化  

Changes in bioaccessibility and antioxidant activity of phenolics in Prunus mume during simulated digestion in vitro

作　　者：杨瑞丽 郭卓钊[1] 施奕乔 郭美媛 黄苇 YANG Ruili;GUO Zhuozhao;SHI Yiqiao;GUO Meiyuan;HUANG Wei(Guangdong Kanghui Group Co.Ltd.,Chaozhou 515638,China;College of Food Science,South China Agricultural University,Guangzhou 510462,China)

机构地区：[1]广东康辉集团有限公司,广东潮州515638 [2]华南农业大学食品学院,广东广州510462

出　　处：《食品与发酵工业》2023年第3期157-163,共7页Food and Fermentation Industries

基　　金：广东省扬帆计划项目(2017YT05H077);国家自然科学基金项目(31871800)。

摘　　要：该研究采用UPLC-MS/MS和INFOGEST 2.0体外静态模拟胃肠道消化模型,分析青梅在消化过程中酚类物质释放量和抗氧化活性的变化,以期探究青梅多酚的消化情况。结果表明,在青梅中共鉴定出10种单体物质,包括7种酚酸和3种黄酮。其中,新绿原酸、表儿茶素、异阿魏酸和p-香豆酸是青梅多酚的主要单体物质。青梅多酚含量为(10.72±0.23)mg GAE/g DW,ABTS和DPPH抗氧化值分别为(16.03±1.00)和(14.65±1.40)mg TE/g DW。青梅多酚的生物可接受率为9.33%。其中,对羟基苯甲酸的生物可接受率(63.20%)最高,其次为芦丁(21.93%)和p-香豆酸(8.14%)。青梅总酚含量、单体酚含量和抗氧化值在体外消化过程中均出现显著下降(P<0.05)。此外,ABTS和DPPH抗氧化值与总酚含量存在显著正相关。其中,原花青素B1、p-香豆酸、阿魏酸和芦丁等单体物质可能是青梅抗氧化能力的主要有效物质。In this study,UPLC-MS/MS and INFOGEST 2.0 were used to statically simulate the gastrointestinal digestion model in vitro to analyze the changes in the release of phenolic substances and antioxidant activities during the digestion process of green plums and to explore the digestion of Prunus mume polyphenols during the digestion process.Results showed that 10 monomers were identified in Prunus mume,including 7 phenolic acids and 3 flavonoids.Among them,neochlorogenic acid,epicatechin,isoferulic acid,and p-coumaric acid were the main monomer substances of Prunus mume polyphenols.The polyphenol content of Prunus mume was(10.72±0.23)mg GAE/g DW,and the antioxidant values of ABTS cationic radicals and DPPH free radicals were(16.03±1.00)mg TE/g DW and(14.65±1.40)mg TE/g DW,respectively.The bioaccessibility rate of polyphenols in Prunus mume was 9.33%.Among them,the bioaccessibility rate of p-hydroxybenzoic acid(63.20%)was the highest,followed by rutin(21.93%)and p-coumaric acid(8.14%).The total phenolic content,monomeric phenolic content,and antioxidant value of Prunus mume were significantly decreased during in vitro digestion(P<0.05).In addition,the antioxidant values of ABTS cationic radicals and DPPH free radicals were significantly positively correlated with the total phenolic content.Among them,individual phenolics such as proanthocyanidin B1,p-coumaric acid,ferulic acid,and rutin may be the main effective substances for the antioxidant capacity of Prunus mume.

关 键 词：青梅 多酚 抗氧化活性 生物可接受率 体外消化 

分 类 号：TS201.2[轻工技术与工程—食品科学]