超高效液相色谱-串联质谱法快速检测白酒与配制酒中8种合成甜味剂  

Determination of 8 artificial sweeteners in liquor and mixed liquor by UPLC-MS/MS

作　　者：周艳华[1] 李涛 卢岚[1] 张春艳[1] ZHOU Yan-hua;LI Tao;LU Lan;ZHANG Chun-yan(Changsha Environmental Protection Vocational College,Changsha 410004,Hunan,China;Hunan Provincial Institute of Product and Goods Quality Inspection,Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning,Changsha 410011,Hunan,China)

机构地区：[1]长沙环境保护职业技术学院,湖南长沙410004 [2]湖南省产商品质量检验研究院,食品安全监测与预警湖南省重点实验室,湖南长沙410111

出　　处：《粮食与油脂》2023年第3期155-158,共4页Cereals & Oils

基　　金：湖南省自然科学基金项目(2022JJ60098);湖南创新型省份建设专项(2020SK2128);湖南省市场监督管理局科技计划项目(2021KJJH46)。

摘　　要：通过超高效液相色谱-串联质谱法建立快速检测白酒和配制酒中8种合成甜味剂的方法,并对8种甜味剂进行基质效应评价和定性定量分析。结果表明:8种合成甜味剂在10.0~200.0 ng/mL内呈良好线性关系,相关系数R^(2)≥0.999,检出限为1.0~14.3μg/kg,定量限为3.3~47.6μg/kg,加标回收率88.2%~111.4%,相对标准偏差(RSD)1.1%~10.0%;白酒基质效应不明显,配制酒中的8种甜味剂基质效应明显,其中安赛蜜基质增强作用最明显,达300.0%;10份白酒样品中检出1份甜蜜素,含量为0.158 mg/kg, 10份配制酒样品中检出1份安赛蜜,含量为0.258 mg/kg, 1份甜蜜素,含量为0.238 mg/kg。该方法简单、快速、高效、准确度高,可作为白酒和配制酒中8种合成甜味剂的定量检测方法。The rapid method for the determination of 8 kinds of synthetic sweeteners in liquor and mixed liquor was established by ultra performance liquid chromatography tandem mass spectrometry. Matrix effects of 8 kinds of sweeteners were evaluated and their qualitative and quantitative analysis were carried out. The results showed that the correlation coefficient was R^(2)≥0.999, the detection limits were 1.0-14.3 μg/kg, the limits of quantification were 3.3-47.6 μg/kg, and the recoveries were 88.2 %-111.4 %, relative standard deviation(RSD)1.1 %-10.0 %. The matrix effect of liquor was not obvious, but 8 kinds of sweetener matrix effect was obvious, among which acesulfamil matrix had the most obvious enhancement effect,(reached 300.0 %). One saccharine content of 0.158 mg/kg was detected in 10 liquor samples, one acesulfamil content of 0.258 mg/kg and one saccharine content of 0.238 mg/kg were detected in 10 mixed liquor samples. The method was simple, rapid, efficient and accurate, and could be used for the quantitative determination of 8 kinds of synthetic sweeteners in liquor and mixed liquor.

关 键 词：超高效液相色谱-串联质谱 甜味剂 白酒 配制酒 

分 类 号：TS207.3[轻工技术与工程—食品科学]