高产开环型monacolin K红曲霉菌株的筛选及其在红曲酒酿造中的应用  

Screening of Monascus spp.strain with high-yield open-loop monacolin K and its application in Hongqujiu brewing

作　　者：郑平 覃先武 张彦[1,2] 刘秀继 栾春艳[1] 陈晖 ZHENG Ping;QIN Xianwu;ZHANG Yan;LIU Xiuji;LUAN Chunyan;CHEN Hui(Hubei Key Laboratory of Yeast Function,Yichang 443003,China;Angel Yeast Co.,Ltd.,Yichang 443003,China)

机构地区：[1]湖北省酵母功能重点实验室,湖北宜昌443003 [2]安琪酵母股份有限公司,湖北宜昌443003

出　　处：《中国酿造》2023年第3期129-134,共6页China Brewing

基　　金：湖北省酵母功能重点实验室项目(A4-101)。

摘　　要：该研究采用38株红曲霉菌株固态发酵制备功能红曲米,采用高效液相色谱法测定红曲米中monacolin K和桔霉素含量,筛选高产开环型莫纳可林K(monacolin K)红曲霉菌株,通过形态观察及分子生物学技术对筛选菌株进行鉴定,并将其应用于红曲酒酿造,分析红曲酒的品质。结果表明,筛选得到1株高产开环型monacolin K、不产桔霉素的菌株ABQ2,经鉴定,其为丛毛红曲霉(Monascus pilosus)。采用该菌株发酵制备功能红曲米中的monacolin K含量达到25.10 mg/g,开环率为90.5%,且桔霉素未检出。采用菌株ABQ2酿造的红曲酒酒精度为12%vol,酸度为1.91 g/100 mL,monacolin K含量为0.48 mg/mL,其理化指标均符合相关行标要求;与小曲酒相比,红曲酒中总酯含量(433.65 mg/L)极显著增加(P<0.01),其中乳酸乙酯和乙酸乙酯含量最高,高级醇类含量(145.52 mg/L)略低,主要为正丙醇、异丁醇、正戊醇、β-苯乙醇等。Functional Monascus rice was prepared using 38 Monascus spp.strains by solid-state fermentation,and the contents of monacolin K and citrinin in Monascus rice were determined by high performance liquid chromatography(HPLC).The Monascus spp.strain with high-yield open-loop monacolin K was screened and identified by morphological observation and molecular biological techniques,it was further used in Hongqujiu brewing,and the quality of Hongqujiu was evaluated.The results showed that,a strain ABQ2 with high-yield open-loop monacolin K and without citrinin yield was screened,which was identified as Monascus pilosus.The content of monacolin K reached 25.10 mg/g in the functional Monascus rice fermented by the strain ABQ2,and the open-loop rate was 90.5%,citrinin was not detected.The alcohol content was 12%vol,acidity was 1.91 g/100 ml,and monacolin K was 0.48 mg/ml in Hongqujiu fermented by the strain ABQ2,its physicochemical indexes were compliance with relevant industrial standards.Compared with Xiaoqujiu,the content of total esters(433.65 mg/L)in Hongqujiu was extremely significantly increased(P<0.01),in which the contents of ethyl lactate and ethyl acetate were the highest,and the content of advanced alcohols(145.52 mg/L)were slightly lower,mainly n-propanol,isobutanol,n-amyl alcohol,β-phenylethanol,etc.

关 键 词：红曲霉 开环型monacolin K 桔霉素 红曲酒 挥发性风味物质 

分 类 号：TS201.3[轻工技术与工程—食品科学]