超声辅助提取玉米黄色素及其抗氧化活性研究  

Study on ultrasound-assisted extraction of corn yellow pigmentand its antioxidant activity

作　　者：马丽媛[1] 石光蕊 尚尔坤 黄雪莹 张晟 李杨[1] 郭丽[1] 王喜庆 MA Liyuan;SHI Guangrui;SHANG Erkun;HUANG Xueying;ZHANG Sheng;LI Yang;GUO Li;WANG Xiqing(School of Food and Pharmaceutical Engineering,Suihua University,Suihua 152061,China;Suihua Inspection and Testing Center,Suihua 152000,China)

机构地区：[1]绥化学院食品与制药工程学院,黑龙江绥化152061 [2]绥化市检验检测中心,黑龙江绥化152000

出　　处：《发酵科技通讯》2023年第2期63-68,共6页Bulletin of Fermentation Science and Technology

基　　金：绥化学院科研创新团队资助项目(SIT05003);黑龙江省大学生创新创业项目(S202210236046);绥化学院2022年社会服务工程项目(SHU2022004)。

摘　　要：以玉米加工副产品玉米皮渣为原料,利用超声波辅助醇提取法提取玉米黄色素。以玉米黄色素的提取率为主要衡量指标,通过单因素实验和正交实验,得到提取玉米黄色素的最佳工艺条件。正交实验结果表明:当超声浸提时间为40 min、料液比为1∶12 g/mL、提取温度为60℃、溶液pH为6时,玉米黄色素最高提取率为6.02%。抗氧化实验结果表明:当样品质量浓度为1,2,3,4,5 mg/mL时,提取的玉米黄色素具有一定的总还原能力、清除DPPH自由基和羟基自由基能力,且这些能力随着样品质量浓度的增加而提高。超声法提取玉米黄色素提取率高,所提取的玉米黄色素具有一定的抗氧化能力。Corn husk residue from the by-product of corn processing was used as the raw material for ultrasonic-assisted ethanol extraction of corn yellow pigment.The extraction rate of corn yellow pigment was selected as the primary measurement index based on a single factor and orthogonal experiments in order to determine the optimal extraction conditions.When the ultrasonic extraction time was 40 min,the material-to-liquid ratio was 1∶12 g/mL,the extraction temperature was 60℃,and the pH value of the solution was 6,the highest extraction rate was 6.02%.The antioxidant activity test revealed that the extracted corn yellow pigment possessed a certain total reducing ability,DPPH and hydroxyl free radical scavenging ability,and that this ability increased with the sample concentration,when the sample concentration was 1~5 mg/mL.It demonstrated that the pigment s extraction rate was high and that it possessed antioxidant properties.

关 键 词：玉米黄色素 超声波 提取率 玉米皮渣 抗氧化活性 

分 类 号：TQ920[轻工技术与工程—发酵工程]